Tang Jing, Quan Yingzhou, Zhang Yueyu, Jiang Min, Al-Enizi Abdullah M, Kong Biao, An Tiance, Wang Wenshuo, Xia Limin, Gong Xingao, Zheng Gengfeng
Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China.
Key Laboratory of Computational Physical Sciences, Ministry of Education, State Key Laboratory of Surface Physics, and Department of Physics, Fudan University, Shanghai 200433, China.
Nanoscale. 2016 Mar 14;8(10):5786-92. doi: 10.1039/c5nr09236a.
Hydrogen peroxide (H2O2) is an important molecular messenger for cellular signal transduction. The capability of direct probing of H2O2 in complex biological systems can offer potential for elucidating its manifold roles in living systems. Here we report the fabrication of three-dimensional (3D) WS2 nanosheet networks with flower-like morphologies on a variety of conducting substrates. The semiconducting WS2 nanosheets with largely exposed edge sites on flexible carbon fibers enable abundant catalytically active sites, excellent charge transfer, and high permeability to chemicals and biomaterials. Thus, the 3D WS2-based nano-bio-interface exhibits a wide detection range, high sensitivity and rapid response time for H2O2, and is capable of visualizing endogenous H2O2 produced in living RAW 264.7 macrophage cells and neurons. First-principles calculations further demonstrate that the enhanced sensitivity of probing H2O2 is attributed to the efficient and spontaneous H2O2 adsorption on WS2 nanosheet edge sites. The combined features of 3D WS2 nanosheet networks suggest attractive new opportunities for exploring the physiological roles of reactive oxygen species like H2O2 in living systems.
过氧化氢（H2O2）是细胞信号转导中的一种重要分子信使。在复杂生物系统中直接探测H2O2的能力为阐明其在生命系统中的多种作用提供了潜力。在这里，我们报告了在各种导电基底上制备具有花状形态的三维（3D）WS2纳米片网络。含有大量暴露边缘位点的半导体WS2纳米片位于柔性碳纤维上，具有丰富的催化活性位点、优异的电荷转移以及对化学物质和生物材料的高渗透性。因此，基于3D WS2的纳米生物界面展现出对H2O2的宽检测范围、高灵敏度和快速响应时间，并且能够可视化活的RAW 264.7巨噬细胞和神经元中产生的内源性H2O2。第一性原理计算进一步表明，探测H2O2灵敏度的提高归因于H2O2在WS2纳米片边缘位点上的高效自发吸附。3D WS2纳米片网络的综合特性为探索像H2O2这样的活性氧在生命系统中的生理作用提供了诱人的新机会。
