Deng Yu, Sun Mingxue, Xu Sha, Zhou Jingwen
National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, Wuxi, Jiangsu, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
J Appl Microbiol. 2016 Jul;121(1):187-95. doi: 10.1111/jam.13105. Epub 2016 May 27.
In order to improve the availability of geranyl diphosphate (GPP) in the mevalonate pathway for enhancing (S)-linalool production in Saccharomyces cerevisiae.
A (S)-linalool synthase (LIS): AaLS1 from Actinidia arguta was coexpressed with FPPS with different peptide linkers to redirect the flux from geranyl diphosphate (GPP) to (S)-linalool production in S. cerevisiae. The strain with the best peptide linker ((GGGGS)3 ), produced 101·55 ± 2·97 μg l(-1) (S)-linalool, a 69·7% increase compared to those with two independent LIS and FPPS expressed. In a 3-l fermenter, the (S)-linalool titre was further improved to 240·64 ± 5·31 μg l(-1) .
The results demonstrate that the fusion proteins catalysing consecutive steps in a metabolic pathway significantly improved the (S)-linalool production with GPP as precursor.
The fusion protein strategy co-expressing AaLS1 and FPPS, assembled with a long peptide linker made S. cerevisiae produced the highest reported (S)-Linalool titre to date.
为了提高甲羟戊酸途径中香叶基二磷酸（GPP）的可用性，以增强酿酒酵母中（S）-芳樟醇的产量。
将来自软枣猕猴桃的（S）-芳樟醇合酶（LIS）：AaLS1与具有不同肽接头的法尼基焦磷酸合酶（FPPS）共表达，以将香叶基二磷酸（GPP）的通量重定向至酿酒酵母中（S）-芳樟醇的生产。具有最佳肽接头（（GGGGS）3）的菌株产生了101.55±2.97μg l-1的（S）-芳樟醇，与单独表达两种LIS和FPPS的菌株相比增加了69.7%。在3-L发酵罐中，（S）-芳樟醇的产量进一步提高到240.64±5.31μg l-1。
结果表明，在代谢途径中催化连续步骤的融合蛋白显著提高了以GPP为前体的（S）-芳樟醇产量。
共表达AaLS1和FPPS并与长肽接头组装的融合蛋白策略使酿酒酵母产生了迄今为止报道的最高（S）-芳樟醇产量。
