Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
Zoonoses Public Health. 2011 Aug;58(5):343-9. doi: 10.1111/j.1863-2378.2010.01353.x. Epub 2010 Sep 17.
Using different typing methods (MLST, spa-, SCCmec- and agr-typing), PFGE and DNA microarray-based chip analysis, we characterized 20 MRSA strains isolated from livestock and veterinarians. PFGE analysis after macrorestriction with EagI provided seven different band patterns, which could be grouped into four clusters. One cluster consisted of all MRSA ST398 strains isolated from pigs, calves, mastitis milk and two veterinarians. One strain of ST398 from a veterinarian and the two strains of ST1 and ST8 formed the three other clusters. Antimicrobial susceptibility testing showed that 15 of 20 strains were resistant to ampicillin, cefoxitin, clindamycin, erythromycin, oxacillin, penicillin and tetracycline. All strains were susceptible to rifampin and vancomycin, 19 were susceptible to ciprofloxacin and 18 were susceptible to sulphamethoxazole/trimethoprim. Genes encoding different enterotoxins, leukotoxins and haemolysins were found in certain strains.
使用不同的分型方法（MLST、spa、SCCmec 和 agr 分型）、PFGE 和基于 DNA 微阵列的芯片分析，我们对从家畜和兽医身上分离出的 20 株 MRSA 菌株进行了特征描述。用 EagI 进行宏观限制后的 PFGE 分析提供了 7 种不同的带型，可以分为 4 个群。一个群由所有从猪、小牛、乳腺炎奶和两名兽医身上分离出的 ST398 型 MRSA 菌株组成。一名兽医身上的一株 ST398 菌株和两株 ST1 和 ST8 菌株形成了另外三个群。药敏试验显示，20 株中有 15 株对氨苄西林、头孢西丁、克林霉素、红霉素、青霉素和四环素耐药。所有菌株均对利福平、万古霉素敏感，19 株对环丙沙星敏感，18 株对磺胺甲噁唑/甲氧苄啶敏感。某些菌株中发现了编码不同肠毒素、白细胞毒素和溶血性毒素的基因。
