Xie Fei, Min Su, Liu Li, Peng Lihua, Hao Xuechao, Zhu Xianlin
Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Arch Gerontol Geriatr. 2016 Jul-Aug;65:1-8. doi: 10.1016/j.archger.2016.02.005. Epub 2016 Feb 15.
The muscle mass decreases with age, leading to frailty and weakness; however, the role of acetylcholine receptors in this process has not been properly studied. In this article, we hypothesize that diaphragmatic as well as peripheral muscle weakness may be caused by the up-regulation of gamma and alpha 7 nicotinic acetylcholine receptors (nAChRs) on muscle cell membranes.
Adult male rats were randomly divided into sham and sepsis groups. Sepsis was induced by cecal ligation and puncture (CLP). Blood specimens and biopsies of tibialis anterior muscle and diaphragm were obtained at 24h post CLP.
Up-regulation of gamma and alpha 7 nAChRs was detected in both sham and septic groups; however, this response was more robust in septic animals. Compared to tibialis anterior muscle, the diaphragm expressed a higher number of both receptor types.
Muscle weakness in old age and sepsis may have common molecular underpinnings. Loss of diaphragmatic strength may explain hypoxia and respiratory failure often encountered in frail elderly.
肌肉质量随年龄增长而下降，导致身体虚弱；然而，乙酰胆碱受体在此过程中的作用尚未得到充分研究。在本文中，我们假设膈肌以及外周肌肉无力可能是由肌细胞膜上γ和α7烟碱型乙酰胆碱受体（nAChRs）上调所致。
成年雄性大鼠随机分为假手术组和脓毒症组。通过盲肠结扎和穿刺（CLP）诱导脓毒症。在CLP术后24小时采集血液样本以及胫骨前肌和膈肌活检样本。
在假手术组和脓毒症组中均检测到γ和α7 nAChRs上调；然而，脓毒症动物中的这种反应更为强烈。与胫骨前肌相比，膈肌中两种受体类型的表达量均更高。
老年和脓毒症导致的肌肉无力可能有共同的分子基础。膈肌力量丧失可能解释了体弱老年人中常见的缺氧和呼吸衰竭。
