Cardozo Tomas Agustina, Ghietto Lucia Maria, Insfran Constanza, Wasinger Nicolas, Marchesi Ariana, Adamo Maria Pilar
Rev Fac Cien Med Univ Nac Cordoba. 2015;72(3):161-9.
Human bocavirus (HBoV) is a parvovirus identified for the first time in 2005 associated to upper- and lower- acute respiratory tract infection (ARI), which is one of the main causes of morbimortality in infant population worldwide. Currently four genotypes have been described named HBoV1-4, of which HBoV1 is the one predominantly related to ARI.
To obtain the complete genome of respiratory HBoV locally isolated.
By means of bioinformatics tools such as ClustalW and NCBI Primer-Blast, primers were designed to amplify overlapping DNA fragments altogether spanning the complete genome of HBoV. Fragments were amplified by PCR and sequenced by BigDye Terminator capillary technology. Sequence editing and phylogenetic analysis were accomplished using MEGA v6 software.
Complete genome sequence of HBoV1 strain 307AR09 was obtained after isolation from respiratory secretion of a pediatric patient with bronchiolitis. The sequence was deposited in the GenBank public database (accession number KJ634207). The phylogenetic analysis including complete genome sequences of all four genotypes from around the world shows similarity close to 100% between the local strain and the virus originally discovered in Sweden (DQ000495). The four genotypes clustered in 2 groups of high internal homology: HBoV1-HBoV3 and HBoV2-HBoV4.
We provide local molecular data that can be used in future technological developments for research and diagnostic tests intended for medical practice. Our results add support to the proposed redistribution of the four genotypes into 2 species.
人博卡病毒（HBoV）是2005年首次鉴定出的一种细小病毒，与上、下呼吸道急性感染（ARI）相关，而ARI是全球婴儿群体发病和死亡的主要原因之一。目前已描述了四种基因型，命名为HBoV1 - 4，其中HBoV1是与ARI主要相关的基因型。
获取本地分离的呼吸道HBoV的完整基因组。
借助ClustalW和NCBI Primer - Blast等生物信息学工具，设计引物以扩增总共覆盖HBoV完整基因组的重叠DNA片段。通过PCR扩增片段，并采用BigDye Terminator毛细管技术进行测序。使用MEGA v6软件完成序列编辑和系统发育分析。
从一名患有细支气管炎的儿科患者的呼吸道分泌物中分离出HBoV1毒株307AR09后，获得了其完整基因组序列。该序列已存入GenBank公共数据库（登录号KJ634207）。包括来自世界各地的所有四种基因型完整基因组序列的系统发育分析表明，本地毒株与最初在瑞典发现的病毒（DQ000495）之间的相似度接近100%。这四种基因型聚为两组，内部同源性高：HBoV1 - HBoV3和HBoV2 - HBoV4。
我们提供了本地分子数据，可用于未来医学实践中研究和诊断测试的技术开发。我们的结果为将四种基因型重新划分为两个物种的提议提供了支持。
