Muzziotti Dayana, Adessi Alessandra, Faraloni Cecilia, Torzillo Giuseppe, De Philippis Roberto
Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale Delle Cascine 24, I 50144, Florence, Italy.
Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale Delle Cascine 24, I 50144, Florence, Italy; Institute of Chemistry of Organometallic Compounds (ICCOM), CNR, Via Madonna del Piano, 10, 50019, Sesto Fiorentino, Florence, Italy.
Res Microbiol. 2016 Jun;167(5):350-6. doi: 10.1016/j.resmic.2016.02.003. Epub 2016 Feb 23.
The ability of coping with the damaging effects of high light intensity represents an essential issue when purple non-sulfur bacteria (PNSB) are grown under direct sunlight for photobiological hydrogen production. This study was aimed at investigating whether H2 photo-evolution could represent, for Rhodopseudomonas palustris 42OL, a safety valve to dissipate an excess of reducing power generated under high light intensities. The physiological status of this strain was assessed under anaerobic (AnG) and aerobic (AG) growing conditions and under H2-producing (HP) conditions at low and high light intensities. The results obtained clearly showed that Fv/Fm ratio was significantly affected by the light intensity under which R. palustris 42OL cells were grown, under either AnG or AG conditions, while, under HP, it constantly remained at its highest value. The increase in light intensity significantly increased the H2 production rate, which showed a positive correlation with the maximum electron transfer rate (rETRmax). These findings are important for optimization of hydrogen production by PNSB under solar light.
当紫色非硫细菌（PNSB）在直射阳光下进行光生物制氢培养时，应对高光强度破坏作用的能力是一个至关重要的问题。本研究旨在调查光驱动产氢是否可以作为沼泽红假单胞菌42OL的一种安全阀，以消散在高光强度下产生的过量还原力。在厌氧（AnG）和好氧（AG）生长条件下以及在低光和高光强度的产氢（HP）条件下评估该菌株的生理状态。所得结果清楚地表明，无论在AnG还是AG条件下，沼泽红假单胞菌42OL细胞生长时的光强度都对Fv/Fm比率有显著影响，而在HP条件下，该比率始终保持在最高值。光强度的增加显著提高了产氢速率，产氢速率与最大电子传递速率（rETRmax）呈正相关。这些发现对于优化PNSB在太阳光下的产氢过程具有重要意义。
