碳水化合物包封对红肉火龙果（Hylocereus polyrhizus (Weber) Britton and Rose）果皮中甜菜色素促健康生物活性的影响

Health-promoting bioactivities of betalains from red dragon fruit (Hylocereus polyrhizus (Weber) Britton and Rose) peels as affected by carbohydrate encapsulation.

作者信息

Rodriguez Evelyn B, Vidallon Mark Louis P, Mendoza David Joram R, Reyes Charisse T

机构信息

Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna 4031, Philippines.

Faculty of Education, University of the Philippines Open University, Los Baños, Laguna 4031, Philippines.

出版信息

J Sci Food Agric. 2016 Nov;96(14):4679-4689. doi: 10.1002/jsfa.7681. Epub 2016 Apr 13.

DOI:10.1002/jsfa.7681

PMID:26916683

Abstract

BACKGROUND

Betalains, which are red-purple and yellow pigments, are ideal alternatives to synthetic colorants as they possess strong coloring potential and excellent health-contributing properties. However, the instability of betalains toward normal storage and biological conditions, in addition to the limited number of betalain sources, impedes their food application and diminishes their bioactivities. This study aimed to evaluate the health-promoting bioactivities of betalains from red dragon fruit (Hylocereus polyrhizus (Weber) Britton and Rose) peels as affected by encapsulation in maltodextrin-gum Arabic and maltodextrin-pectin matrices.

RESULTS

Encapsulation in maltodextrin-gum Arabic and maltodextrin-pectin matrices afforded dry betalain powders after lyophilization. Optical microscopy imaging showed that the betalain powders consisted of matrix-type and shard-like microparticles. ABTS antioxidant assay revealed that maltodextrin-gum Arabic-betalain (MGB) and maltodextrin-pectin-betalain (MPB) microparticles possessed higher antioxidant capacities (195.39 ± 8.63 and 201.76 ± 4.06 µmol Trolox g microparticles respectively) than the non-encapsulated betalain extract (151.07 ± 2.57 µmol Trolox g extract). Duck embryo chorioallantoic membrane (CAM) vascular irritation assay showed that the anti-inflammatory activity of encapsulated betalains was five- to six-fold higher than that of non-encapsulated betalains (P ≤ 0.05). Antiangiogenic activity, as evaluated by duck embryo CAM assay, was enhanced two- to four-fold by carbohydrate encapsulation. Glutathione S-transferase (GST)-inducing activity of betalains was likewise improved four- to five-fold.

CONCLUSION

The study showed that the antioxidant, anti-inflammatory, antiangiogenic and GST-inducing activities of betalains from red dragon fruit peels were enhanced through carbohydrate encapsulation. © 2016 Society of Chemical Industry.

摘要

背景

甜菜色素是红紫色和黄色色素，具有很强的着色潜力和出色的健康促进特性，是合成色素的理想替代品。然而，甜菜色素在正常储存和生物条件下不稳定，且甜菜色素来源有限，这阻碍了它们在食品中的应用，并降低了它们的生物活性。本研究旨在评估经麦芽糊精 - 阿拉伯胶和麦芽糊精 - 果胶基质包封后，红肉火龙果（Hylocereus polyrhizus (Weber) Britton and Rose）果皮中甜菜色素的健康促进生物活性。

结果

经麦芽糊精 - 阿拉伯胶和麦芽糊精 - 果胶基质包封后，冻干得到了干燥的甜菜色素粉末。光学显微镜成像显示，甜菜色素粉末由基质型和碎片状微粒组成。ABTS抗氧化试验表明，麦芽糊精 - 阿拉伯胶 - 甜菜色素（MGB）和麦芽糊精 - 果胶 - 甜菜色素（MPB）微粒具有比未包封的甜菜色素提取物更高的抗氧化能力（分别为195.39 ± 8.63和201.76 ± 4.06 μmol Trolox/g微粒）（未包封的甜菜色素提取物为151.07 ± 2.57 μmol Trolox/g提取物）。鸭胚绒毛尿囊膜（CAM）血管刺激试验表明，包封后的甜菜色素的抗炎活性比未包封的甜菜色素高五到六倍（P≤0.05）。通过鸭胚CAM试验评估，碳水化合物包封使抗血管生成活性提高了两到四倍。甜菜色素的谷胱甘肽S - 转移酶（GST）诱导活性同样提高了四到五倍。