Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Int J Biol Macromol. 2013 Jul;58:18-22. doi: 10.1016/j.ijbiomac.2013.03.041. Epub 2013 Mar 25.
The polysaccharides extracted by hot water and precipitated by 50% ethanol from the fruiting bodies of cultured Cordyceps militaris (W-CBP50) were introduced. Antioxidant activities of W-CBP50, W-CBP50 I (obtained from W-CBP50 purified by Sephadex G-100 chromatography) and W-CBP50 II (obtained from W-CBP50I purified by Sephadex G-200 chromatography) were evaluated and structural features of W-CBP50 II were analyzed. The polysaccharide W-CBP50 II was able to scavenge the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), while W-CBP50 and W-CBP50I could scavenge DPPH, hydroxyl and superoxide radical. Structural characteristics of W-CBP50 II were investigated using GPC, GC-MS, (1)H NMR and IR. The results showed that it was mainly composed of α-glucose, α-mannose, α-galactose and α-arabinose with α-type glycosidic linkage and the molecular weight of its main component was 8.97 kD.
从培养的蛹虫草子实体中热水提取、50%乙醇沉淀得到的多糖（W-CBP50）被介绍。评估了 W-CBP50、W-CBP50 I（从 Sephadex G-100 层析纯化的 W-CBP50 获得）和 W-CBP50 II（从 Sephadex G-200 层析纯化的 W-CBP50 I 获得）的抗氧化活性，并分析了 W-CBP50 II 的结构特征。多糖 W-CBP50 II 能够清除稳定自由基 1,1-二苯基-2-苦基肼（DPPH），而 W-CBP50 和 W-CBP50 I 则能够清除 DPPH、羟基和超氧自由基。使用 GPC、GC-MS、（1）H NMR 和 IR 研究了 W-CBP50 II 的结构特征。结果表明，它主要由α-葡萄糖、α-甘露糖、α-半乳糖和α-阿拉伯糖组成，具有α型糖苷键，其主要成分的分子量为 8.97 kD。
