Bauer B A, Rogers P J, Miller T D, Bove A A, Tyce G M
Division of Cardiovascular Diseases and Internal Medicine, Mayo Clinic, Rochester, MN 55905.
Med Sci Sports Exerc. 1989 Oct;21(5):558-62.
In dogs the concentrations of conjugated dopamine in plasma have previously been shown to increase after exercise training. This study was done to determine whether conjugated norepinephrine and epinephrine also increase. Fifteen dogs were randomly divided into training (N = 8) or sedentary (N = 7) groups. All dogs were exercised acutely for 5 min at 4 mph with a 12% grade, following a 3-min warm-up, before and after either a 12-wk training or a 12-wk sedentary period. Free and conjugated catecholamines were determined in blood drawn at rest and during acute exercise using high-performance liquid chromatography (HPLC) with electrochemical detection. Before training, free norepinephrine, epinephrine, dopamine, and conjugated norepinephrine increased in plasma during acute exercise. Following the 12-wk training period, there were significant increases in free and conjugated dopamine and in conjugated norepinephrine in plasma taken at rest. There were no such increases in resting catecholamines after a 12-wk sedentary period. After either training or sedentary periods, dogs responded to acute exercise with an increase in free norepinephrine and a decrease in conjugated norepinephrine. Thus, after training both conjugated norepinephrine and dopamine, but not conjugated epinephrine, increased in plasma. The data suggest that sulfation of catecholamines increases as a result of exercise training.
先前的研究表明，犬类在运动训练后血浆中结合多巴胺的浓度会升高。本研究旨在确定结合去甲肾上腺素和肾上腺素是否也会升高。15只犬被随机分为训练组（N = 8）或久坐组（N = 7）。在进行12周的训练或12周的久坐期之前和之后，所有犬在3分钟热身之后，以4英里/小时的速度、12%的坡度进行5分钟的急性运动。使用高效液相色谱法（HPLC）和电化学检测法测定静息和急性运动期间采集的血液中的游离和结合儿茶酚胺。训练前，急性运动期间血浆中的游离去甲肾上腺素、肾上腺素、多巴胺和结合去甲肾上腺素会升高。在12周的训练期后，静息时采集的血浆中游离和结合多巴胺以及结合去甲肾上腺素显著增加。在12周的久坐期后，静息儿茶酚胺没有这种增加。在训练期或久坐期后，犬对急性运动的反应是游离去甲肾上腺素增加，结合去甲肾上腺素减少。因此，训练后血浆中结合去甲肾上腺素和多巴胺增加，但结合肾上腺素没有增加。数据表明，运动训练会导致儿茶酚胺的硫酸化增加。
