Scalize Priscilla Hakime, Bombonato-Prado Karina F, de Sousa Luiz Gustavo, Rosa Adalberto Luiz, Beloti Marcio Mateus, Semprini Marisa, Gimenes Rossano, de Almeida Adriana L G, de Oliveira Fabíola Singaretti, Hallak Regalo Simone Cecilio, Siessere Selma
Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
Federal University of Itajubá (UNIFEI), Itajubá, Minas Gerais, Brazil.
J Mater Sci Mater Med. 2016 Dec;27(12):180. doi: 10.1007/s10856-016-5799-x. Epub 2016 Oct 21.
Osteoporosis is a chronic disease that impairs proper bone remodeling. Guided bone regeneration is a surgical technique that improves bone defect in a particular region through new bone formation, using barrier materials (e.g. membranes) to protect the space adjacent to the bone defect. The polytetrafluorethylene membrane is widely used in guided bone regeneration, however, new membranes are being investigated. The purpose of this study was to evaluate the effect of P(VDFTrFE)/BT [poly(vinylidene fluoride-trifluoroethylene)/barium titanate] membrane on in vivo bone formation. Twenty-three Wistar rats were submitted to bilateral ovariectomy. Five animals were subjected to sham surgery. After 150 days, bone defects were created and filled with P(VDF-TrFE)/BT membrane or PTFE membrane (except for the sham and OVX groups). After 4 weeks, the animals were euthanized and calvaria samples were subjected to histomorphometric and computed microtomography analysis (microCT), besides real time polymerase chain reaction (real time PCR) to evaluate gene expression. The histomorphometric analysis showed that the animals that received the P(VDF-TrFE)/BT membrane presented morphometric parameters similar or even better compared to the animals that received the PTFE membrane. The comparison between groups showed that gene expression of RUNX2, BSP, OPN, OSX and RANKL were lower on P(VDF-TrFE)/BT membrane; the gene expression of ALP, OC, RANK and CTSK were similar and the gene expression of OPG, CALCR and MMP9 were higher when compared to PTFE. The results showed that the P(VDF-TrFE)/BT membrane favors bone formation, and therefore, may be considered a promising biomaterial to support bone repair in a situation of osteoporosis.
骨质疏松症是一种损害正常骨重塑的慢性疾病。引导性骨再生是一种外科技术，通过使用屏障材料（如膜）保护骨缺损邻近区域的空间，促进特定区域的骨缺损通过新骨形成得到改善。聚四氟乙烯膜在引导性骨再生中被广泛应用，然而，新型膜材料仍在研究中。本研究的目的是评估聚（偏二氟乙烯-三氟乙烯）/钛酸钡（P(VDF-TrFE)/BT）膜对体内骨形成的影响。23只Wistar大鼠接受双侧卵巢切除术。5只动物接受假手术。150天后，制造骨缺损并用P(VDF-TrFE)/BT膜或聚四氟乙烯膜填充（假手术组和卵巢切除组除外）。4周后，对动物实施安乐死，取颅盖骨样本进行组织形态计量学和计算机断层扫描分析（显微CT），并进行实时聚合酶链反应（实时PCR）以评估基因表达。组织形态计量学分析表明，接受P(VDF-TrFE)/BT膜的动物与接受聚四氟乙烯膜的动物相比，呈现出相似甚至更好的形态计量学参数。组间比较显示，与聚四氟乙烯膜相比，P(VDF-TrFE)/BT膜上RUNX2、骨涎蛋白（BSP）、骨桥蛋白（OPN）、osterix（OSX）和核因子κB受体活化因子配体（RANKL）的基因表达较低；碱性磷酸酶（ALP）、骨钙素（OC）、RANK和组织蛋白酶K（CTSK）的基因表达相似，骨保护素（OPG）、钙敏感受体（CALCR）和基质金属蛋白酶9（MMP9）的基因表达较高。结果表明，P(VDF-TrFE)/BT膜有利于骨形成，因此，在骨质疏松症情况下，可被视为一种有前景的支持骨修复的生物材料。
