Morais Sergi, Puchades Rosa, Maquieira Ángel
Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Camino de Vera s/n, 46071, Valencia, Spain.
Anal Bioanal Chem. 2016 Jul;408(17):4523-34. doi: 10.1007/s00216-016-9423-1. Epub 2016 Feb 27.
The idea of using disk drives to monitor molecular biorecognition events on regular optical discs has received considerable attention during the last decade. CDs, DVDs, Blu-ray discs and other new optical discs are universal and versatile supports with the potential for development of protein and DNA microarrays. Besides, standard disk drives incorporated in personal computers can be used as compact and affordable optical reading devices. Consequently, a CD technology, resulting from the audio-video industry, has been used to develop analytical applications in health care, environmental monitoring, food safety and quality assurance. The review presents and critically evaluates the current state of the art of disc-based microarrays with illustrative examples, including past, current and future developments. Special mention is made of the analytical developments that use either chemically activated or raw standard CDs where proteins, oligonucleotides, peptides, haptens or other biological probes are immobilized. The discs are also used to perform the assays and must maintain their readability with standard optical drives. The concept and principle of evolving disc-based microarrays and the evolution of disk drives as optical detectors are also described. The review concludes with the most relevant uses ordered chronologically to provide an overview of the progress of CD technology applications in the life sciences. Also, it provides a selection of important references to the current literature. Graphical Abstract High density disc-based microarrays.
在过去十年中，利用磁盘驱动器监测常规光盘上的分子生物识别事件这一想法受到了广泛关注。CD、DVD、蓝光光盘及其他新型光盘是通用且多功能的载体，具有开发蛋白质和DNA微阵列的潜力。此外，个人电脑中配备的标准磁盘驱动器可用作紧凑且经济实惠的光学读取设备。因此，源自音频视频行业的CD技术已被用于开发医疗保健、环境监测、食品安全和质量保证等领域的分析应用。本综述通过实例展示并批判性地评估了基于光盘的微阵列的当前技术水平，包括过去、现在和未来的发展情况。特别提及了使用化学活化或原始标准CD的分析进展，这些CD上固定有蛋白质、寡核苷酸、肽、半抗原或其他生物探针。这些光盘还用于进行检测，并且必须能够通过标准光驱保持其可读性。文中还描述了不断发展的基于光盘的微阵列的概念和原理，以及作为光学探测器的磁盘驱动器的演变。综述按时间顺序列出了最相关的用途，以概述CD技术在生命科学领域应用的进展情况。此外，还提供了当前文献的重要参考文献选集。图形摘要：高密度基于光盘的微阵列。
