Kikuchi Mari, Konno Hiroyuki
a Department of Biochemical Engineering, Graduate School of Science and Technology , Yamagata University , Yonezawa , Japan.
Biosci Biotechnol Biochem. 2016 Jun;80(6):1066-9. doi: 10.1080/09168451.2016.1148581. Epub 2016 Feb 29.
The cytotoxic evaluation of natural and synthetic callipeltins is described. Cyclic depsipeptide callipeltin B and linear peptides callipeltins E and M synthesized by us showed no cytotoxic activity. In contrast, natural callipeltin B purified from Callipelta sp. showed CC50 = 130 μM against Hela cells. We found that purified callipeltin B included the contamination of callipeltins C and H at a ratio of approximately 15%. These results suggested that the cytotoxicity of natural callipeltin B was derived from callipeltins C and H.
描述了天然和合成的卡里佩尔汀的细胞毒性评估。我们合成的环状缩肽卡里佩尔汀B以及线性肽卡里佩尔汀E和M均未显示出细胞毒性活性。相比之下，从卡里佩尔塔属物种中纯化得到的天然卡里佩尔汀B对Hela细胞显示出CC50 = 130 μM。我们发现纯化的卡里佩尔汀B中含有约15%比例的卡里佩尔汀C和H的污染物。这些结果表明天然卡里佩尔汀B的细胞毒性源自卡里佩尔汀C和H。
