Zendehdel M, Baghbanzadeh A, Aghelkohan P, Hassanpour S
a Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine , University of Tehran , 14155-6453 , Tehran , Iran.
b Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch , Islamic Azad University , Tehran , Iran.
Br Poult Sci. 2016 Apr;57(2):271-9. doi: 10.1080/00071668.2016.1141173.
The aim of the current study was to investigate the interaction of the lipopolysaccharide (LPS) and histaminergic systems on appetite regulation in broilers. Effects of intracerebroventricular (ICV) injection of α-fluoromethylhistidine (α-FMH, histidine decarboxylase inhibitor), chlorpheniramine (histamine H1 receptor antagonist), famotidine (histamine H2 receptor antagonist) and thioperamide (histamine H3 receptor antagonist) on LPS-induced hypophagia in broilers were studied. A total of 128 broilers were randomly allocated into 4 experiments (4 groups and 8 replications in each experiment). A cannula was surgically implanted into the lateral ventricle. In Experiment 1, broilers were ICV injected with LPS (20 ng) prior to α-FMH (250 nmol). In Experiment 2, chickens were ICV injected with LPS followed by chlorpheniramine (300 nmol). In Experiment 3, broilers were ICV injected with famotidine (82 nmol) after LPS (20 ng). In Experiment 4, ICV injection of LPS was followed by thioperamide (300 nmol). Then, cumulative food intake was recorded until 4 h post-injection. According to the results, LPS significantly decreased food intake. Chlorpheniramine significantly amplified food intake, and LPS-induced hypophagia was lessened by injection of chlorpheniramine. α-FMH, famotidine and thioperamide had no effect on LPS-induced hypophagia. These results suggest that there is an interaction between central LPS and the histaminergic system where LPS-induced hypophagia is mediated by H1 histamine receptors in 3 h food-deprived broilers.
本研究的目的是探讨脂多糖（LPS）与组胺能系统在肉鸡食欲调节中的相互作用。研究了脑室内（ICV）注射α-氟甲基组氨酸（α-FMH，组氨酸脱羧酶抑制剂）、氯苯那敏（组胺H1受体拮抗剂）、法莫替丁（组胺H2受体拮抗剂）和硫代哌酰胺（组胺H3受体拮抗剂）对LPS诱导的肉鸡摄食减少的影响。总共128只肉鸡被随机分为4个实验（每个实验4组，每组8个重复）。通过手术将套管植入侧脑室。在实验1中，在注射α-FMH（250 nmol）之前，给肉鸡脑室内注射LPS（20 ng）。在实验2中，给鸡脑室内注射LPS，随后注射氯苯那敏（300 nmol）。在实验3中，给肉鸡脑室内注射LPS（20 ng）后，注射法莫替丁（82 nmol）。在实验4中，脑室内注射LPS后，注射硫代哌酰胺（300 nmol）。然后，记录注射后4小时内的累积食物摄入量。结果显示，LPS显著降低了食物摄入量。氯苯那敏显著增加了食物摄入量，注射氯苯那敏减轻了LPS诱导的摄食减少。α-FMH、法莫替丁和硫代哌酰胺对LPS诱导的摄食减少没有影响。这些结果表明，中枢LPS与组胺能系统之间存在相互作用，在禁食3小时的肉鸡中，LPS诱导的摄食减少是由H1组胺受体介导的。
