Li Chuting, Liu Yuan, Liu Dexiang, Jiang Hong, Pan Fang
Department of Medical Psychology, Shandong University School of Medicine, No. 44 Wenhua Xi Road, Jinan, Shandong 250012, China.
Neural Plast. 2016;2016:5249893. doi: 10.1155/2016/5249893. Epub 2016 Jan 26.
Several types of microRNA (miRNA) overexpression in the brain are associated with stress. One of the targets of miR-34c is the stress-related corticotrophin releasing factor receptor 1 mRNA (CRFR1 mRNA). Here we will probe into the short-term effect and long-term effect of early adolescent traumatic stress on the expression of miR-34c and CRFR1 mRNA. Traumatic stress was established by electric foot shock for six consecutive days using 28-day rats. The anxiety-like behaviors, memory damage, CRFR1 protein, CRFR1 mRNA, and miR-34c expression were detected in our study. The results of our study proved that exposure to acute traumatic stress in early adolescent can cause permanent changes in neural network, resulting in dysregulation of CRFR1 expression and CRFR1 mRNA and miR-34c expression in hypothalamus, anxiety-like behavior, and memory impairment, suggesting that the miR-34c expression in hypothalamus may be an important factor involved in susceptibility to PTSD.
大脑中几种类型的微小RNA（miRNA）过表达与应激有关。miR-34c的靶标之一是与应激相关的促肾上腺皮质激素释放因子受体1 mRNA（CRFR1 mRNA）。在此，我们将探究青少年早期创伤应激对miR-34c和CRFR1 mRNA表达的短期和长期影响。采用28日龄大鼠，通过连续6天的足部电击建立创伤应激模型。在我们的研究中检测了焦虑样行为、记忆损伤、CRFR1蛋白、CRFR1 mRNA和miR-34c表达。我们的研究结果证明，青少年早期暴露于急性创伤应激可导致神经网络的永久性变化，从而导致下丘脑CRFR1表达、CRFR1 mRNA和miR-34c表达失调、焦虑样行为和记忆障碍，提示下丘脑miR-34c表达可能是参与创伤后应激障碍易感性的一个重要因素。
