用噬菌体进行鼻内治疗可使小鼠从鲍曼不动杆菌介导的肺炎中获救。
Intranasal treatment with bacteriophage rescues mice from Acinetobacter baumannii-mediated pneumonia.
作者信息
Wang Yong, Mi Zhiqiang, Niu Wenkai, An Xiaoping, Yuan Xin, Liu Huiying, Li Puyuan, Liu Yannan, Feng Yuzhong, Huang Yong, Zhang Xianglilan, Zhang Zhiyi, Fan Hang, Peng Fan, Tong Yigang, Bai Changqing
机构信息
Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
出版信息
Future Microbiol. 2016 May;11:631-41. doi: 10.2217/fmb.16.11. Epub 2016 Feb 29.
AIM
With the emergence of drug-resistant bacteria, finding alternative agents to treat antibiotic-resistant bacterial infections is imperative.
MATERIALS & METHODS: A mouse pneumonia model was developed by combining cyclophosphamide pretreatment and Acinetobacter baumannii challenge, and a lytic bacteriophage was evaluated for its therapeutic efficacy in this model by examining the survival rate, bacterial load in the lung and lung pathology.
RESULTS
Intranasal instillation with bacteriophage rescued 100% of mice following lethal challenge with A. baumannii. Phage treatment reduced bacterial load in the lung. Microcomputed tomography indicated a reduction in lung inflammation in mice given phage.
CONCLUSION
This research demonstrates that intranasal application of bacteriophage is viable, and could provide complete protection from pneumonia caused by A. baumannii.
目的
随着耐药细菌的出现，寻找替代药物治疗耐抗生素细菌感染势在必行。
材料与方法
通过环磷酰胺预处理和鲍曼不动杆菌攻击建立小鼠肺炎模型，并通过检测存活率、肺内细菌载量和肺部病理学来评估一种裂解性噬菌体在该模型中的治疗效果。
结果
用噬菌体滴鼻给药可使遭受鲍曼不动杆菌致死性攻击的小鼠100%存活。噬菌体治疗降低了肺内细菌载量。微型计算机断层扫描显示接受噬菌体治疗的小鼠肺部炎症减轻。
结论
本研究表明，经鼻应用噬菌体是可行的，并且可以为鲍曼不动杆菌引起的肺炎提供完全保护。
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