基于质谱的蛋白质翻译后修饰蛋白质组学分析
Proteomic Analysis of Protein Posttranslational Modifications by Mass Spectrometry.
作者信息
Swaney Danielle L, Villén Judit
机构信息
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
出版信息
Cold Spring Harb Protoc. 2016 Mar 1;2016(3):pdb.top077743. doi: 10.1101/pdb.top077743.
Abstract
The addition of posttranslational modifications (PTMs) to proteins is an influential mechanism to temporally control protein function and ultimately regulate entire cellular processes. Most PTMs are present at low stoichiometry and abundance, which limits their detection when analyzing whole cell lysates. PTM purification methods are thus required to comprehensively characterize the presence and dynamics of PTMs using mass spectrometry-based proteomics approaches. Here we describe several of the most influential PTMs and discuss the fundamentals of proteomics experiments and PTM purification methods.
摘要
对蛋白质进行翻译后修饰（PTM）是一种在时间上控制蛋白质功能并最终调节整个细胞过程的重要机制。大多数翻译后修饰的化学计量和丰度较低，这限制了在分析全细胞裂解物时对它们的检测。因此，需要采用翻译后修饰纯化方法，通过基于质谱的蛋白质组学方法全面表征翻译后修饰的存在和动态变化。在此，我们描述几种最具影响力的翻译后修饰，并讨论蛋白质组学实验和翻译后修饰纯化方法的基本原理。
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本文引用的文献
1
Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.蛋白质降解中磷酸化和泛素化相互作用的全局分析。
Nat Methods. 2013 Jul;10(7):676-82. doi: 10.1038/nmeth.2519. Epub 2013 Jun 9.
2
Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.蛋白质组范围内赖氨酸乙酰化分析表明其在酿酒酵母中广泛的调控范围。
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. doi: 10.1074/mcp.M112.017251. Epub 2012 Aug 2.
3
Global identification of modular cullin-RING ligase substrates.全局鉴定模块化 Cullin-RING 连接酶底物。
Cell. 2011 Oct 14;147(2):459-74. doi: 10.1016/j.cell.2011.09.019. Epub 2011 Sep 29.
4
Systematic and quantitative assessment of the ubiquitin-modified proteome.系统且定量的泛素化蛋白质组学研究。
Mol Cell. 2011 Oct 21;44(2):325-40. doi: 10.1016/j.molcel.2011.08.025. Epub 2011 Sep 8.
5
A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.一种基于蛋白质组的、定量的体内泛素化位点调查揭示了广泛的调节作用。
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. doi: 10.1074/mcp.M111.013284. Epub 2011 Sep 1.
6
PhosphoGRID: a database of experimentally verified in vivo protein phosphorylation sites from the budding yeast Saccharomyces cerevisiae.磷酸化蛋白质组数据库（PhosphoGRID）：一个从酿酒酵母（Saccharomyces cerevisiae）中提取的、经实验验证的体内蛋白质磷酸化位点数据库。
Database (Oxford). 2010;2010:bap026. doi: 10.1093/database/bap026. Epub 2010 Jan 28.
7
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.信号网络中的全局、体内及位点特异性磷酸化动力学
Cell. 2006 Nov 3;127(3):635-48. doi: 10.1016/j.cell.2006.09.026.
8
A proteomics approach to understanding protein ubiquitination.一种用于理解蛋白质泛素化的蛋白质组学方法。
Nat Biotechnol. 2003 Aug;21(8):921-6. doi: 10.1038/nbt849. Epub 2003 Jul 20.
9
Proteomic analysis of post-translational modifications.翻译后修饰的蛋白质组学分析
Nat Biotechnol. 2003 Mar;21(3):255-61. doi: 10.1038/nbt0303-255.
10
Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.通过质谱法进行磷酸化蛋白质组分析及其在酿酒酵母中的应用。
Nat Biotechnol. 2002 Mar;20(3):301-5. doi: 10.1038/nbt0302-301.
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