Hashemian Maryam, Poustchi Hossein, Pourshams Akram, Khoshnia Masoud, Brockman John Douglas, Hekmatdoost Azita, Abnet Christian C, Malekzadeh Reza
Department of Nutrition, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran ; Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Middle East J Dig Dis. 2016 Jan;8(1):19-23. doi: 10.15171/mejdd.2016.02.
BACKGROUND In the Golestan Cohort Study that was launched to investigate the causes of esophageal cancer, a complete biospecimen bank was established for storage of collected blood, urine, hair, and nail samples. The objective of this study was to evaluate the feasibility of nail samples as a biomarker of selected trace elements status. METHODS Thirty toenail samples were selected randomly from the participants of Golestan Cohort Study (GCS). The samples were cleaned and analyzed for selenium, mercury, chromium, iron, zinc, and scandium by instrumental neutron activation analysis at the University of Missouri Research Reactor Center. Pearson correlation coefficients were computed for selected trace elements concentration versus scandium concentration to assess terrestrial contamination. RESULTS The selenium, zinc, and mercury were not correlated with scandium, suggesting they were free from substantial contamination. The high correlations of scandium with iron and chromium suggest that the iron and chromium levels may be compromised by terrestrial contamination. The coefficients of variation for duplicate samples for selenium and zinc were 2.6% and 7.2%, respectively. CONCLUSION The nail samples from Golestan Cohort Study appears to be useable as a biomarker of selenium, zinc, and mercury and could be considered for use in future studies.
背景 在旨在调查食管癌病因的戈勒斯坦队列研究中，建立了一个完整的生物样本库，用于储存采集的血液、尿液、毛发和指甲样本。本研究的目的是评估指甲样本作为所选微量元素状态生物标志物的可行性。方法 从戈勒斯坦队列研究（GCS）的参与者中随机选取30份 toenail 样本。样本经过清洗，并在密苏里大学研究反应堆中心通过仪器中子活化分析测定其中的硒、汞、铬、铁、锌和钪。计算所选微量元素浓度与钪浓度的皮尔逊相关系数，以评估地面污染情况。结果 硒、锌和汞与钪不相关，表明它们没有受到大量污染。钪与铁和铬的高度相关性表明，铁和铬的水平可能受到地面污染的影响。硒和锌重复样本的变异系数分别为2.6%和7.2%。结论 戈勒斯坦队列研究的指甲样本似乎可用作硒、锌和汞的生物标志物，可考虑在未来研究中使用。
