Bheema Lingeswara Reddy I N, Chandrasekhar K, Zewdu Y, Dinoor A, Keller B, Ben-David R
Department of Vegetables and Field Crops, Institute of Plant Sciences, Agricultural Research Organization (ARO)-Volcani Center, 5025000, Bet Dagan, Israel.
Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
Theor Appl Genet. 2016 Jun;129(6):1127-37. doi: 10.1007/s00122-016-2688-0. Epub 2016 Mar 2.
Gene PmAF7DS confers resistance to wheat powdery mildew (isolate Bgt#211 ); it was mapped to a 14.6-cM interval ( Xgwm350 a- Xbarc184 ) on chromosome 7DS. The flanking markers could be applied in MAS breeding. Wheat powdery mildew (Pm) is caused by the biotrophic pathogen Blumeria graminis tritici (DC.) (Bgt). An ongoing threat of breakdown of race-specific resistance to Pm requires a continuous effort to discover new alleles in the wheat gene pool. Developing new cultivars with improved disease resistance is an economically and environmentally safe approach to reduce yield losses. To identify and characterize genes for resistance against Pm in bread wheat we used the (Arina × Forno) RILs population. Initially, the two parental lines were screened with a collection of 61 isolates of Bgt from Israel. Three Pm isolates Bgt#210 , Bgt#211 and Bgt#213 showed differential reactions in the parents: Arina was resistant (IT = 0), whereas Forno was moderately susceptible (IT = -3). Isolate Bgt#211 was then used to inoculate the RIL population. The segregation pattern of plant reactions among the RILs indicates that a single dominant gene controls the conferred resistance. A genetic map of the region containing this gene was assembled with DNA markers and assigned to the 7D physical bin map. The gene, temporarily designated PmAF7DS, was located in the distal region of chromosome arm 7DS. The RILs were also inoculated with Bgt#210 and Bgt#213. The plant reactions to these isolates showed high identity with the reaction to Bgt#211, indicating the involvement of the same gene or closely linked, but distinct single genes. The genomic location of PmAF7DS, in light of other Pm genes on 7DS is discussed.
基因PmAF7DS赋予小麦对白粉病（分离株Bgt#211）的抗性；它被定位到7DS染色体上一个14.6厘摩的区间（Xgwm350a - Xbarc184）。侧翼标记可应用于标记辅助选择育种。小麦白粉病（Pm）由活体营养型病原菌小麦白粉菌（DC.）（Bgt）引起。针对Pm的小种特异性抗性存在失效的持续威胁，这就需要不断努力在小麦基因库中发现新的等位基因。培育具有更强抗病性的新品种是减少产量损失的一种经济且环境友好的方法。为了鉴定和表征面包小麦中抗Pm的基因，我们使用了（阿瑞纳×福尔诺）重组自交系群体。最初，用来自以色列的61个Bgt分离株对两个亲本系进行了筛选。三个Pm分离株Bgt#210、Bgt#211和Bgt#213在亲本中表现出不同反应：阿瑞纳具有抗性（侵染型 = 0），而福尔诺中度感病（侵染型 = -3）。然后使用分离株Bgt#211接种重组自交系群体。重组自交系中植株反应的分离模式表明，一个单一显性基因控制着所赋予的抗性。利用DNA标记构建了包含该基因区域的遗传图谱，并将其定位到7D物理图谱单元上。该基因暂时命名为PmAF7DS，位于7DS染色体臂的远端区域。还用Bgt#210和Bgt#213接种了重组自交系。植株对这些分离株的反应与对Bgt#211的反应高度一致，表明涉及同一个基因或紧密连锁但不同的单个基因。结合7DS上的其他Pm基因，讨论了PmAF7DS的基因组定位。
