Yamamoto Shoko, Deguchi Kisaburo, Onuma Masamichi, Numata Noriaki, Sakai Yasuo
Central Research Institute, Jellice Co., Ltd.
Biol Pharm Bull. 2016;39(3):428-34. doi: 10.1248/bpb.b15-00624.
Collagen tripeptide (CTP) is a collagen hydrolysate containing a high concentration of tripeptides with a Gly-X-Y sequence, such as Gly-Pro-Hyp. To test the effects of this preparation, we compared the absorption of peptides in humans after ingestion of a tripeptide fraction of CTP (CTP-100), a CTP preparation containing ca. 50% Gly-X-Y tripeptides (CTP-50), and a collagen peptide that did not contain tripeptides (CP). The postprandial levels of Gly-Pro-Hyp and Pro-Hyp in the plasma increased in those subjects who ingested CTP-100 and CTP-50, and were higher with greater Gly-Pro-Hyp ingestion. This demonstrated that collagen hydrolysates were efficiently absorbed when the collagen was ingested in the tripeptide form. Gly-Pro-Hyp and Pro-Hyp were also found in the urine after ingestion of CTP-100 or CTP-50. Similar to the results for the plasma concentration, the urinary excretion of Gly-Pro-Hyp and Pro-Hyp was also dependent on the amount of Gly-Pro-Hyp ingested. This indicates that ingested Gly-Pro-Hyp and generated Pro-Hyp were relatively stable in the body and were transported to the urine in the peptide form. The concentration of Hyp-Gly in the plasma was low after the ingestion of CP and CTP-100 but higher after the ingestion of CTP-50. Overall, our results suggest that tripeptides derived from collagen are absorbed efficiently by the body.
胶原蛋白三肽（CTP）是一种胶原蛋白水解产物，含有高浓度的具有甘氨酸- X - Y序列的三肽，如甘氨酸-脯氨酸-羟脯氨酸。为了测试这种制剂的效果，我们比较了人类摄入CTP的三肽部分（CTP - 100）、含有约50%甘氨酸- X - Y三肽的CTP制剂（CTP - 50）和不含三肽的胶原蛋白肽（CP）后肽的吸收情况。摄入CTP - 100和CTP - 50的受试者血浆中甘氨酸-脯氨酸-羟脯氨酸和脯氨酸-羟脯氨酸的餐后水平升高，且摄入的甘氨酸-脯氨酸-羟脯氨酸越多，水平越高。这表明当胶原蛋白以三肽形式摄入时，胶原蛋白水解产物能被有效吸收。摄入CTP - 100或CTP - 50后，尿液中也发现了甘氨酸-脯氨酸-羟脯氨酸和脯氨酸-羟脯氨酸。与血浆浓度结果相似，甘氨酸-脯氨酸-羟脯氨酸和脯氨酸-羟脯氨酸的尿排泄也取决于摄入的甘氨酸-脯氨酸-羟脯氨酸量。这表明摄入的甘氨酸-脯氨酸-羟脯氨酸和生成的脯氨酸-羟脯氨酸在体内相对稳定，并以肽的形式转运到尿液中。摄入CP和CTP - 100后血浆中羟脯氨酸-甘氨酸的浓度较低，但摄入CTP - 50后较高。总体而言，我们的结果表明，源自胶原蛋白的三肽能被人体有效吸收。
