Martínez-Ibáñez M, Juan-Díaz M J, Lara-Saez I, Coso A, Franco J, Gurruchaga M, Suay Antón J, Goñi Isabel
Polymer Science and Technology Department, University of the Basque Country (UPV/EHU), P Manuel de Lardizabal, 3, 20018, San Sebastián, Spain.
Industrial Systems Engineering and Design Department, Jaime I University (UJI), 12071, Castellón de la Plana, Spain.
J Mater Sci Mater Med. 2016 Apr;27(4):80. doi: 10.1007/s10856-016-5690-9. Epub 2016 Mar 2.
Taking into account the influence of Si in osteoblast cell proliferation, a series of sol-gel derived silicon based coating was prepared by controlling the process parameters and varying the different Si-alkoxide precursors molar rate in order to obtain materials able to release Si compounds. For this purpose, methyltrimethoxysilane (MTMOS) and tetraethyl orthosilicate (TEOS) were hydrolysed together and the sol obtained was used to dip-coat the different substrates. The silicon release ability of the coatings was tested finding that it was dependent on the TEOS precursor content, reaching a Si amount value around ninefolds higher for coatings with TEOS than for the pure MTMOS material. To test the effect of this released Si, the in vitro performance of developed coatings was tested with human adipose mesenchymal stem cells finding a significantly higher proliferation and mineralization on the coating with the higher TEOS content. For in vivo evaluation of the biocompatibility, coated implants were placed in the tibia of the rabbit and a histological analysis was performed. The evaluation of parameters such as the bone marrow state, the presence of giant cells and the fibrous capsule proved the biocompatibility of the developed coatings. Furthermore, coated implants seemed to produce a qualitatively higher osteoblastic activity and a higher number of bone spicules than the control (uncoated commercial SLA titanium dental implant).
考虑到硅对成骨细胞增殖的影响，通过控制工艺参数并改变不同硅醇盐前驱体的摩尔比，制备了一系列溶胶-凝胶法衍生的硅基涂层，以获得能够释放硅化合物的材料。为此，将甲基三甲氧基硅烷（MTMOS）和正硅酸四乙酯（TEOS）一起水解，所得溶胶用于浸涂不同的基材。测试了涂层的硅释放能力，发现其取决于TEOS前驱体的含量，含TEOS的涂层的硅释放量比纯MTMOS材料高出约九倍。为了测试这种释放的硅的效果，用人类脂肪间充质干细胞测试了所制备涂层的体外性能，发现TEOS含量较高的涂层上细胞增殖和矿化明显更高。为了对生物相容性进行体内评估，将涂覆的植入物植入兔子的胫骨中并进行了组织学分析。对骨髓状态、巨细胞的存在和纤维囊等参数的评估证明了所制备涂层的生物相容性。此外，与对照（未涂覆的商用SLA钛牙科植入物）相比，涂覆的植入物似乎产生了更高质量的成骨细胞活性和更多的骨针。
