磷酸改性钛表面的表面特性及早期小鼠前成骨细胞反应
Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface.
作者信息
Osathanon Thanaphum, Sawangmake Chenphop, Ruangchainicom Nanticha, Wutikornwipak Pavitra, Kantukiti Panisa, Nowwarote Nunthawan, Pavasant Prasit
机构信息
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
J Oral Biol Craniofac Res. 2016 Jan-Apr;6(1):2-9. doi: 10.1016/j.jobcr.2015.12.005. Epub 2015 Dec 24.
AIMS
The present study investigated the surface properties and murine pre-osteoblast cell (MC3T3-E1) responses of phosphoric acid (H3PO4) treated commercially pure titanium.
METHODS
Titanium discs were treated with various concentration of H3PO4 (5%, 10%, and 20%; v/v) at 90 °C for 30 min. Surface properties were evaluated by profilometer, contact angle meter, and scanning electron microscopy (SEM) with energy dispersive X-rays. MC3T3-E1 attachment and spreading were evaluated by SEM and phalloidin immunohistochemistry staining.
RESULTS
Surface roughness and wettability were not statistically difference among all experimental and control groups. Phosphate and oxygen were detected on H3PO4 treated surfaces. At 20 min, cell attachment was significantly higher in 10% and 20% H3PO4 treated groups compared to the control. Cells exhibited orientated-cytoskeleton fibers on 20% H3PO4 modified titanium surface. Though, there was no difference in cell spreading stage among all treatment groups.
CONCLUSION
H3PO4 treatment on titanium may influence early cell response, particularly on attachment and spreading.
目的
本研究调查了磷酸（H3PO4）处理的商业纯钛的表面特性和小鼠前成骨细胞（MC3T3-E1）反应。
方法
将钛盘在90°C下用不同浓度的H3PO4（5%、10%和20%；v/v）处理30分钟。通过轮廓仪、接触角测量仪以及带有能量色散X射线的扫描电子显微镜（SEM）评估表面特性。通过SEM和鬼笔环肽免疫组织化学染色评估MC3T3-E1细胞的附着和铺展情况。
结果
所有实验组和对照组之间的表面粗糙度和润湿性无统计学差异。在H3PO4处理过的表面检测到了磷酸盐和氧。在20分钟时，10%和20% H3PO4处理组的细胞附着明显高于对照组。在20% H3PO4改性钛表面，细胞呈现出定向的细胞骨架纤维。不过，所有处理组之间的细胞铺展阶段没有差异。
结论
对钛进行H3PO4处理可能会影响细胞早期反应，特别是在附着和铺展方面。
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