Tashakori-Sabzevar Faezeh, Ramezani Masoud, Hosseinzadeh Hossein, Parizadeh Seyyed Mohammad Reza, Movassaghi Ahmad Reza, Ghorbani Ahmad, Mohajeri Seyed Ahmad
Student Research Committee (SRC), Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Acta Diabetol. 2016 Aug;53(4):609-19. doi: 10.1007/s00592-016-0842-4. Epub 2016 Mar 4.
Diabetes mellitus is a major manifestation of metabolic disorder which presents with hyperglycemia (high levels of serum blood sugar). In the present study, we aimed to investigate the effects of celery seed extract on different biochemical factors and histopathological changes in normal and streptozotocin (STZ)-induced diabetic rats.
A total of 35 male Wistar rats were divided into five groups (one normal and four diabetic groups). STZ was injected intraperitoneally to induce diabetes. The effects of hexane extract of celery seed and glibenclamide (as a positive control) were compared. Blood samples were analyzed on days 0, 18, and 33, and histopathological evaluations were performed at the end of the study.
Glucose, triglycerides, and cholesterol levels significantly decreased, whereas insulin and high-density lipoprotein (HDL) levels increased in the extract-administered groups, as compared to the negative diabetic control group (P < 0.0001). The concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of the extract-administered groups were significantly less than the negative control group (P < 0.0001). Histopathological reports revealed significantly less atrophy, necrosis, and inflammation in the rats receiving celery seed extract compared to the negative control group.
The results indicated that celery seed extract can be effective in controlling hyperglycemia and hyperlipidemia in diabetic rats, and demonstrated its protective effects against pancreatic toxicity resulting from STZ-induction.
糖尿病是代谢紊乱的主要表现，其特征为高血糖（血清血糖水平升高）。在本研究中，我们旨在探讨芹菜籽提取物对正常及链脲佐菌素（STZ）诱导的糖尿病大鼠不同生化因子及组织病理学变化的影响。
总共35只雄性Wistar大鼠被分为五组（一组正常组和四组糖尿病组）。通过腹腔注射STZ诱导糖尿病。比较芹菜籽己烷提取物和格列本脲（作为阳性对照）的效果。在第0天、18天和33天采集血样进行分析，并在研究结束时进行组织病理学评估。
与糖尿病阴性对照组相比，提取物给药组的血糖、甘油三酯和胆固醇水平显著降低，而胰岛素和高密度脂蛋白（HDL）水平升高（P < 0.0001）。提取物给药组血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的浓度显著低于阴性对照组（P < 0.0001）。组织病理学报告显示，与阴性对照组相比，接受芹菜籽提取物的大鼠萎缩、坏死和炎症明显减轻。
结果表明，芹菜籽提取物可有效控制糖尿病大鼠的高血糖和高脂血症，并证明其对STZ诱导的胰腺毒性具有保护作用。
