Ramani Vijay, Shendure Jay
Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA.
Howard Hughes Medical Institute, 3720 15th Ave NE, Seattle, WA, 98195, USA.
Genome Biol. 2016 Mar 5;17:42. doi: 10.1186/s13059-016-0905-4.
DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
德里西及其同事展示了Cas9在体外的一种创造性应用，即利用它去除DNA文库中不需要的序列。CRISPR/Cas9在体外的应用似乎有尚未实现的潜力，能给分子生物学实践带来变革，且远不止于基因组编辑。
