Klimentov Alexander S, Butenko Alexander M, Khutoretskaya Natalia V, Shustova Elena Yu, Larichev Victor F, Isaeva Olga V, Karganova Galina G, Lukashev Alexander N, Gmyl Anatoly P
Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia; D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
J Virol Methods. 2016 Jun;232:29-32. doi: 10.1016/j.jviromet.2016.02.009. Epub 2016 Mar 4.
This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.
本研究报告了一种能够检测白蛉/蚊子传播和蜱传播的静脉病毒的全静脉病毒检测方法。使用一组虫媒病毒验证了该检测方法的灵敏度和特异性。逆转录聚合酶链反应（RT-PCR）检测方法简单且灵敏，因此非常适合用于野外样本的筛查。
