Britsch S, Christ B, Jacob H J
Institut für Anatomie der Ruhr Universität Bochum, Federal Republic of Germany.
Anat Embryol (Berl). 1989;180(5):479-84. doi: 10.1007/BF00305123.
The extracellular matrix-component fibronectin (FN) was detected in close localisation to the vascular system (VS) of the quail chorioallantoic membrane (CAM). We have examined the role of cell-fibronectin interactions within the developing CAM. In two series of experiments the CAM was directly exposed to (1) an antibody against the cell-binding fragment of FN, and to (2) RGD-containing synthetic peptides which are recognized by the FN receptor. For controls an antibody against tubulin and a SHLVE-pentapeptide that does not interfere with the FN-receptor were applied. In the presence of anti-FN antibodies and RGD-sequences the CAM could not establish a normal vascular system. We observed hypo- and partially avascular regions; the resulting vascular pattern was atypically lacunar. None of the control substances affected the regular development of the chorioallantoic vascular system. These results demonstrate the essential role of FN in CAM angiogenesis.
在鹌鹑尿囊绒毛膜（CAM）的血管系统（VS）附近检测到细胞外基质成分纤连蛋白（FN）。我们研究了发育中的CAM内细胞与纤连蛋白相互作用的作用。在两个系列的实验中，将CAM直接暴露于（1）抗FN细胞结合片段的抗体，以及（2）被FN受体识别的含RGD的合成肽。作为对照，应用了抗微管蛋白抗体和不干扰FN受体的SHLVE五肽。在存在抗FN抗体和RGD序列的情况下，CAM无法建立正常的血管系统。我们观察到血管减少和部分无血管区域；形成的血管模式呈非典型腔隙状。对照物质均未影响尿囊绒毛膜血管系统的正常发育。这些结果证明了FN在CAM血管生成中的重要作用。
