Su Yarui, Zhang Dale, Li Yuge, Li Suoping
Laboratory of Plant Germplasm Resources and Genetic Engineering, Henan University, Kaifeng, PR China.
Cytogenet Genome Res. 2015;147(4):268-73. doi: 10.1159/000444435. Epub 2016 Mar 8.
Intergeneric hybrids and amphidiploid hybrids from crosses of Aegilopstauschii and Secale cereale were produced using young embryo rescue. The hybrids showed complete sets of both parental chromosomes. The dihaploid plants showed an average meiotic pairing configuration of 10.84 I + 1.57 II + 0.01 III. Genomic in situ staining revealed 3 types of bivalent associations, i.e. D-D, R-R and D-R at frequencies of 8.6, 8.2 and 83.3%, respectively. Trivalents consisted of D-R-D or R-D-R associations. These results suggested that both intra- and intergenomic chromosome homology were contributed to chromosome pairing. Derived amphidiploids with 2n = 28 paired at metaphase I of meiosis as 4.51 I + 11.70 II + 0.03 III. Chromosome pairing of amphidiploids appeared more or less regular, i.e. bivalent-like with some trivalent configurations.
利用幼胚拯救技术获得了节节麦与黑麦杂交的属间杂种和双二倍体杂种。这些杂种具有双亲的全套染色体。双单倍体植株减数分裂配对构型平均为10.84Ⅰ+1.57Ⅱ+0.01Ⅲ。基因组原位杂交显示有3种二价体联会类型，即D-D、R-R和D-R，频率分别为8.6%、8.2%和83.3%。三价体由D-R-D或R-D-R联会组成。这些结果表明，基因组内和基因组间的染色体同源性都对染色体配对有贡献。衍生的2n = 28的双二倍体在减数分裂中期Ⅰ配对为4.51Ⅰ+11.70Ⅱ+0.03Ⅲ。双二倍体的染色体配对或多或少是规则的，即类似二价体并带有一些三价体构型。
