Ding Xie-Gang, Li Shi-Wen, Zheng Xin-Min, Wang Xing-Huan, Luo Yi
Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
Asian J Androl. 2017 May-Jun;19(3):298-302. doi: 10.4103/1008-682X.175780.
In this study, we investigated the feasibility of using autologous vein graft and platelet-derived growth factors to bridge transected cavernous nerve in a rat model. A short defect in the bilateral cavernous nerve was created and repaired with vein graft from the right jugular vein or vein graft plus platelet-derived growth factors. The 32 rats were divided into four groups, namely Group 1 - no repair as a negative control, Group 2 - vein graft alone, Group 3 - vein graft plus platelet-derived growth factors, and Group 4 - sham operation as a positive control. We evaluated nerve regeneration and functional recovery using retrograde tracing study with FluoroGold, Toluidine blue staining of cavernous nerve, and the intracavernous pressure at 3 months. Three months after surgery, rich FluoroGold-positive cells were observed in the sham and vein graft plus platelet-derived growth factors group, but very few were found in the no repair group. The number of myelinated axons of regenerated cavernous nerve and intracavernous pressure were increased obviously in the two vein graft groups, especially in the vein graft plus platelet-derived growth factors group. These findings confirm the feasibility of using autologous vein as guides for cavernous nerve regeneration, and the regeneration can be further enhanced when the vein is filled with platelet-derived growth factors.
在本研究中，我们在大鼠模型中研究了使用自体静脉移植物和血小板衍生生长因子桥接横断的海绵体神经的可行性。制造双侧海绵体神经的短缺损，并用来自右颈静脉的静脉移植物或静脉移植物加血小板衍生生长因子进行修复。32只大鼠分为四组，即第1组 - 不修复作为阴性对照，第2组 - 单独静脉移植物，第3组 - 静脉移植物加血小板衍生生长因子，第4组 - 假手术作为阳性对照。我们使用FluoroGold逆行示踪研究、海绵体神经甲苯胺蓝染色以及3个月时的海绵体内压来评估神经再生和功能恢复。术后3个月，在假手术组和静脉移植物加血小板衍生生长因子组中观察到丰富的FluoroGold阳性细胞，但在不修复组中发现很少。两个静脉移植物组中再生海绵体神经的有髓轴突数量和海绵体内压明显增加，尤其是在静脉移植物加血小板衍生生长因子组中。这些发现证实了使用自体静脉作为海绵体神经再生引导物的可行性，并且当静脉填充有血小板衍生生长因子时，再生可以进一步增强。
