Li Xinfei, Zhao Lin, Adam Mohamed
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China.
Mar Pollut Bull. 2016 Apr 15;105(1):43-50. doi: 10.1016/j.marpolbul.2016.02.073. Epub 2016 Mar 4.
This study aims at constructing an efficient bacterial consortium to biodegrade crude oil spilled in China's Bohai Sea. In this study, TCOB-1 (Ochrobactrum), TCOB-2 (Brevundimonas), TCOB-3 (Brevundimonas), TCOB-4 (Bacillus) and TCOB-5 (Castellaniella) were isolated from Bohai Bay. Through the analysis of hydrocarbon biodegradation, TCOB-4 was found to biodegrade more middle-chain n-alkanes (from C17 to C23) and long-chain n-alkanes (C31-C36). TCOB-5 capable to degrade more n-alkanes including C24-C30 and aromatics. On the basis of complementary advantages, TCOB-4 and TCOB-5 were chosen to construct a consortium which was capable of degrading about 51.87% of crude oil (2% w/v) after 1week of incubation in saline MSM (3% NaCl). It is more efficient compared with single strain. In order to biodegrade crude oil, the construction of bacterial consortia is essential and the principle of complementary advantages could reduce competition between microbes.
本研究旨在构建一个高效的细菌群落，用于生物降解在中国渤海海域泄漏的原油。在本研究中，TCOB - 1（苍白杆菌属）、TCOB - 2（短波单胞菌属）、TCOB - 3（短波单胞菌属）、TCOB - 4（芽孢杆菌属）和TCOB - 5（卡氏菌属）从渤海湾分离得到。通过对烃类生物降解的分析，发现TCOB - 4能降解更多的中链正构烷烃（C17至C23）和长链正构烷烃（C31 - C36）。TCOB - 5能够降解更多包括C24 - C30的正构烷烃和芳烃。基于互补优势，选择TCOB - 4和TCOB - 5构建一个细菌群落，该群落能够在含3% NaCl的盐渍无机盐培养基中培养1周后降解约51.87%的原油（2% w/v）。与单一菌株相比，其效率更高。为了生物降解原油，构建细菌群落至关重要，且优势互补原则可减少微生物之间的竞争。
