Hiroe M, Hirata Y, Marumo F, Nagata M, Toyozaki T, Hasumi M, Ohta Y, Horie T, Sekiguchi M
Department of Radiology and Cardiology, Tokyo Women's Medical College, Japan.
Peptides. 1989 Nov-Dec;10(6):1281-2. doi: 10.1016/0196-9781(89)90021-1.
The cellular localization of endothelin (ET), a novel vasoconstrictor peptide, was studied in human vascular tissues by immunohistochemistry. Distinct and diffuse staining for ET-like immunoreactivity was demonstrated in the cytoplasm of vascular endothelial cells, but not in smooth muscle cells or adventitial fibroblasts. The specificity was confirmed by the negative results following immunoabsorption. These findings suggest that human vascular endothelial cells function as an endocrine and/or paracrine cells for ET secretion.
采用免疫组织化学方法，对一种新型血管收缩肽内皮素（ET）在人体血管组织中的细胞定位进行了研究。在血管内皮细胞的细胞质中呈现出ET样免疫反应的明显且弥漫性染色，但在平滑肌细胞或外膜成纤维细胞中未观察到。免疫吸附后的阴性结果证实了其特异性。这些发现表明，人体血管内皮细胞可作为分泌ET的内分泌和/或旁分泌细胞发挥作用。
