定量磷酸化蛋白质组学的当前技术水平及其在糖尿病研究中的应用。
The current state of the art of quantitative phosphoproteomics and its applications to diabetes research.
作者信息
Chan Chi Yuet X'avia, Gritsenko Marina A, Smith Richard D, Qian Wei-Jun
机构信息
a Biological Sciences Division and Environmental Molecular Sciences Laboratory , Pacific Northwest National Laboratory , Richland , WA , USA.
出版信息
Expert Rev Proteomics. 2016;13(4):421-33. doi: 10.1586/14789450.2016.1164604.
Abstract
Protein phosphorylation is a fundamental regulatory mechanism in many cellular processes and aberrant perturbation of phosphorylation has been implicated in various human diseases. Kinases and their cognate inhibitors have been considered as hotspots for drug development. Therefore, the emerging tools, which enable a system-wide quantitative profiling of phosphoproteome, would offer a powerful impetus in unveiling novel signaling pathways, drug targets and/or biomarkers for diseases of interest. This review highlights recent advances in phosphoproteomics, the current state of the art of the technologies and the challenges and future perspectives of this research area. Finally, some exemplary applications of phosphoproteomics in diabetes research are underscored.
摘要
蛋白质磷酸化是许多细胞过程中的一种基本调节机制，磷酸化的异常扰动与多种人类疾病有关。激酶及其同源抑制剂一直被视为药物开发的热点。因此，能够对磷酸化蛋白质组进行全系统定量分析的新兴工具，将为揭示感兴趣疾病的新信号通路、药物靶点和/或生物标志物提供强大动力。本综述重点介绍了磷酸化蛋白质组学的最新进展、技术的当前水平以及该研究领域的挑战和未来展望。最后，强调了磷酸化蛋白质组学在糖尿病研究中的一些典型应用。
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