Li Qian, Wu Weiyun, Lin Huandong, Chang Xinxia, Bian Hua, Xia Mingfeng, Yan Hongmei, Gao Xin
Department of Endocrinology and Metabolism, Shanghai Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Department of Clinical Laboratory, Shanghai Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Lipids Health Dis. 2016 Mar 9;15:52. doi: 10.1186/s12944-016-0215-6.
The association between serum Retinol Binding Protein 4 (RBP4) and obesity is still controversial. Serum RBP4 levels varies by gender, and estradiol may play a role in the difference. To investigate the participation of sex hormones in the association of RBP4 and obesity in humans, we measured serum RBP4, BMI, and sex hormones in 87 women from the outpatient.
Eighty-seven subjects of Chinese women origin from the outpatient (aged 40.22 ± 15.54 years) were enrolled. Subjects with diseases affecting the metabolic state or not suitable to participate in this study were excluded. Anthropometrics and laboratory tests, including lipid profile, luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), estradiol (E2),progesterone (PROG), testosterone (TESTO), and dehydroepiandrosterone (DHEA) were conducted. Serum RBP4 was detected by an enzyme immunoassay kit and validated by quantitative Western blotting.
Circulating RBP4 levels were positively associated with BMI, waist circumference, waist-to-hip ratio (WHR), systolic and diastolic (SBP), diastolic blood pressure (DBP), triglycerides (TG), low high-density lipoprotein cholesterol(LDL-c), and testosterone (TESTO) in the total group. While only in obese individuals, serum RBP4 levels were negatively associated with E2. The highest value was in the subjects with both obesity and the low estrogen level. Multiple linear regression analysis revealed that RBP4 correlated independently with TG, TC and insulin in all subjects, TC in non- obese individuals. However, E2 were significantly associated with serum RBP4 only in obese individuals.
RBP4 could be a marker of obesity-related factors; estrogen was negatively related to RBP4 and might be one of the influential factors.
血清视黄醇结合蛋白4（RBP4）与肥胖之间的关联仍存在争议。血清RBP4水平因性别而异，雌二醇可能在这种差异中起作用。为了研究性激素在人类RBP4与肥胖关联中的作用，我们测量了门诊87名女性的血清RBP4、体重指数（BMI）和性激素水平。
招募了87名来自门诊的中国女性受试者（年龄40.22±15.54岁）。排除患有影响代谢状态疾病或不适合参与本研究的受试者。进行了人体测量和实验室检测，包括血脂谱、促黄体生成素（LH）、促卵泡生成素（FSH）、催乳素（PRL）、雌二醇（E2）、孕酮（PROG）、睾酮（TESTO）和脱氢表雄酮（DHEA）。血清RBP4通过酶免疫分析试剂盒检测，并通过定量蛋白质免疫印迹法进行验证。
在整个研究组中，循环RBP4水平与BMI、腰围、腰臀比（WHR）、收缩压和舒张压（SBP、DBP）、甘油三酯（TG）、低高密度脂蛋白胆固醇（LDL-c）以及睾酮（TESTO）呈正相关。而仅在肥胖个体中，血清RBP4水平与E2呈负相关。肥胖且雌激素水平低的受试者中该值最高。多元线性回归分析显示，在所有受试者中，RBP4与TG、总胆固醇（TC）和胰岛素独立相关，在非肥胖个体中与TC独立相关。然而，E2仅在肥胖个体中与血清RBP4显著相关。
RBP4可能是肥胖相关因素的一个标志物；雌激素与RBP4呈负相关，可能是影响因素之一。
