单纯疱疹病毒、巨细胞病毒和爱泼斯坦-巴尔病毒在结直肠癌中的显示
Demonstration of Herpes Simplex Virus, Cytomegalovirus, and Epstein-Barr Virus in Colorectal Cancer.
作者信息
Mehrabani-Khasraghi Sahar, Ameli Mitra, Khalily Farzad
机构信息
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran.
Department of Medicine, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran.
出版信息
Iran Biomed J. 2016 Nov;20(5):302-6. doi: 10.22045/ibj.2016.08. Epub 2016 May 25.
Abstract
BACKGROUND
The present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in CRC and colorectal polyp by using the PCR method in Iran.
METHODS
In this analytical case-control study, we selected 15 patients with CRC, 20 patients with colorectal polyp, and 35 patients without malignancy as controls. After DNA extraction, PCR was used to determine HSV, CMV, and EBV genome by specific primers. Statistical analysis was performed using χ2 tests.
RESULTS
Our findings demonstrated that there is no direct molecular evidence to support the association between HSV, CMV, and EBV and human colorectal malignancies.
CONCLUSION
The results from this study do not exclude a possible oncogenic role of these viruses in neoplastic development of colon cells.
摘要
背景
本研究旨在通过聚合酶链反应（PCR）方法，调查伊朗结直肠癌（CRC）和大肠息肉中单纯疱疹病毒（HSV）、巨细胞病毒（CMV）和爱泼斯坦-巴尔病毒（EBV）存在情况之间关联的分子证据。
方法
在这项分析性病例对照研究中，我们选取了15例CRC患者、20例大肠息肉患者以及35例无恶性肿瘤患者作为对照。提取DNA后，使用特异性引物通过PCR检测HSV、CMV和EBV基因组。采用χ2检验进行统计分析。
结果
我们的研究结果表明，没有直接的分子证据支持HSV、CMV、EBV与人类结直肠癌之间存在关联。
结论
本研究结果并未排除这些病毒在结肠细胞肿瘤发生发展中可能的致癌作用。
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