College of Medicine, QU Health, Qatar University, Doha 2713, Qatar.
Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha 2713, Qatar.
Int J Mol Sci. 2021 Jul 29;22(15):8118. doi: 10.3390/ijms22158118.
Colorectal cancer (CRC) is the third most common cause of cancer-related deaths worldwide. Human papillomaviruses (HPVs) and Epstein-Barr virus (EBV) have been reported to be present in different types of human cancers, including CRCs, where they can play a key role in the onset and/or progression of these cancers. Thus, we herein explored the prevalence of high-risk HPVs and EBV in a cohort of 94 CRC tissue samples and 13 colorectal normal tissues from the Lebanese population using polymerase chain reaction, immunohistochemistry, and tissue microarray methodologies. We found that high-risk HPVs are present in 64%, while EBV is present in 29% of our CRC samples. Additionally, our data showed that high-risk HPV types (16, 18, 35, 58, 51, 45, 52, 31, and 33) are the most frequent in CRC in the Lebanese cohort, respectively. Our data point out that HPVs and EBV are copresent in 28% of the samples. Thus, this study clearly suggests that high-risk HPVs and EBV are present/copresent in CRCs, where they could play an important role in colorectal carcinogenesis. Nevertheless, further investigations using a larger cohort are needed to elucidate the possible cooperation between these oncoviruses in the development of CRC.
结直肠癌（CRC）是全球癌症相关死亡的第三大常见原因。人乳头瘤病毒（HPV）和 Epstein-Barr 病毒（EBV）已被报道存在于不同类型的人类癌症中，包括 CRC，它们在这些癌症的发生和/或进展中可能发挥关键作用。因此，我们使用聚合酶链反应、免疫组织化学和组织微阵列方法，在黎巴嫩人群的 94 个 CRC 组织样本和 13 个结直肠正常组织中探索了高危 HPV 和 EBV 的流行情况。我们发现高危 HPV 存在于 64%的 CRC 样本中，而 EBV 存在于 29%的 CRC 样本中。此外，我们的数据显示，高危 HPV 类型（16、18、35、58、51、45、52、31 和 33）分别是黎巴嫩队列中 CRC 中最常见的类型。我们的数据指出，HPV 和 EBV 在 28%的样本中同时存在。因此，本研究清楚地表明，高危 HPV 和 EBV 存在于 CRC 中，它们可能在结直肠癌变中发挥重要作用。然而，需要进一步使用更大的队列进行研究，以阐明这些致癌病毒在 CRC 发展过程中的可能合作。
