Nehlin J O, Carlberg M, Ronne H
Ludwig Institute for Cancer Research, Uppsala Branch, Sweden.
Gene. 1989 Dec 28;85(2):313-9. doi: 10.1016/0378-1119(89)90423-x.
We have cloned and sequenced the GAL2 gene of Saccharomyces cerevisiae, which encodes galactose permease. The GAL2 protein is related to the yeast glucose transporter encoded by the SNF3 gene, and also to mammalian and bacterial sugar permeases. Like the other members of this protein family, GAL2 has twelve hydrophobic segments that are separated by loops of charged amino acids. A comparison of different members of this protein family shows that those parts of the polypeptides thought to be on the cytoplasmic side of the cell membrane, are more conserved than other parts of the molecules.
我们已经克隆并测序了酿酒酵母的GAL2基因，该基因编码半乳糖通透酶。GAL2蛋白与由SNF3基因编码的酵母葡萄糖转运蛋白相关，也与哺乳动物和细菌的糖通透酶相关。与该蛋白家族的其他成员一样，GAL2有12个疏水片段，这些片段被带电荷氨基酸的环分隔开。对该蛋白家族不同成员的比较表明，那些被认为位于细胞膜细胞质一侧的多肽部分，比分子的其他部分更保守。
