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GAL2编码酿酒酵母中半乳糖通透酶的一个膜结合亚基。

GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.

作者信息

Tschopp J F, Emr S D, Field C, Schekman R

出版信息

J Bacteriol. 1986 Apr;166(1):313-8. doi: 10.1128/jb.166.1.313-318.1986.

DOI:10.1128/jb.166.1.313-318.1986
PMID:3082856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214594/
Abstract

The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.

摘要

克隆了酿酒酵母半乳糖通透酶（GAL2）的编码基因。该克隆可使gal2酵母恢复半乳糖通透酶活性，并且其受半乳糖调控的方式与其他GAL基因产物（GAL1、-7和-10）相似。对温度条件性分泌突变体的实验表明，GAL2基因产物转运至细胞表面需要功能正常的分泌途径。此外，构建了GAL2基因与大肠杆菌lacZ基因之间的基因融合体。GAL2-lacZ基因融合体在酵母中编码受半乳糖调控的β-半乳糖苷酶活性。发现β-半乳糖苷酶活性与膜结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c933/214594/d45c069ab866/jbacter00209-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c933/214594/d45c069ab866/jbacter00209-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c933/214594/d45c069ab866/jbacter00209-0324-a.jpg

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