Sutton Patrick L, Luo Zunping, Divis Paul C S, Friedrich Volney K, Conway David J, Singh Balbir, Barnwell John W, Carlton Jane M, Sullivan Steven A
Center for Genomics and Systems Biology, Department of Biology, New York University, 12 Waverly Place, New York, NY 10003, United States.
Pathogen Molecular Biology Department, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom; Malaria Research Centre, Faculty of Medicine and Health Sciences, University Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Infect Genet Evol. 2016 Jun;40:243-252. doi: 10.1016/j.meegid.2016.03.009. Epub 2016 Mar 14.
Plasmodium cynomolgi is a malaria parasite that typically infects Asian macaque monkeys, and humans on rare occasions. P. cynomolgi serves as a model system for the human malaria parasite Plasmodium vivax, with which it shares such important biological characteristics as formation of a dormant liver stage and a preference to invade reticulocytes. While genomes of three P. cynomolgi strains have been sequenced, genetic diversity of P. cynomolgi has not been widely investigated. To address this we developed the first panel of P. cynomolgi microsatellite markers to genotype eleven P. cynomolgi laboratory strains and 18 field isolates from Sarawak, Malaysian Borneo. We found diverse genotypes among most of the laboratory strains, though two nominally different strains were found to be genetically identical. We also investigated sequence polymorphism in two erythrocyte invasion gene families, the reticulocyte binding protein and Duffy binding protein genes, in these strains. We also observed copy number variation in rbp genes.
食蟹猴疟原虫是一种通常感染亚洲猕猴的疟原虫，偶尔也会感染人类。食蟹猴疟原虫是人类疟原虫间日疟原虫的模型系统，它与间日疟原虫具有重要的生物学特征，如形成休眠肝期和偏好侵入网织红细胞。虽然已经对三种食蟹猴疟原虫菌株的基因组进行了测序，但食蟹猴疟原虫的遗传多样性尚未得到广泛研究。为了解决这个问题，我们开发了第一组食蟹猴疟原虫微卫星标记，对11株食蟹猴疟原虫实验室菌株和来自马来西亚婆罗洲沙捞越的18株野外分离株进行基因分型。我们在大多数实验室菌株中发现了不同的基因型，不过发现两个名义上不同的菌株在基因上是相同的。我们还研究了这些菌株中两个红细胞入侵基因家族，即网织红细胞结合蛋白和达菲结合蛋白基因的序列多态性。我们还观察到rbp基因的拷贝数变异。
