反义非编码RNA与基因转录调控
Antisense non-coding RNAs and regulation of gene transcription.
作者信息
Beltran Manuel, García de Herreros Antonio
机构信息
a UCL Cancer Institute, University College of London , London , United Kingdom.
b Programa de Recerca en Càncer, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM) , Barcelona , Spain.
出版信息
Transcription. 2016 Mar 14;7(2):39-43. doi: 10.1080/21541264.2016.1148804. Epub 2016 Mar 17.
Abstract
Transcriptome analyses have revealed the existence of a large variety of non-coding RNAs (ncRNAs) that, although specifically expressed, are largely unknown in function. The best-studied role of ncRNAs is the regulation of gene expression, mostly participating in transcription. We report here the role of an antisense ncRNA that represses transcription of LEF1 by recruiting Polycomb repressive complex 2 (PRC2), which trimethylates H3K27. This antisense LEF1 transcript undergoes splicing losing its inhibitory function. We also discuss the possible general relevance of this finding.
摘要
转录组分析揭示了大量非编码RNA（ncRNA）的存在，这些RNA虽然有特异性表达，但其功能大多未知。ncRNA研究得最透彻的作用是基因表达调控，主要参与转录过程。我们在此报告一种反义ncRNA的作用，它通过招募多梳抑制复合物2（PRC2）来抑制LEF1的转录，PRC2会使H3K27发生三甲基化。这种反义LEF1转录本会进行剪接，从而失去其抑制功能。我们还讨论了这一发现可能具有的普遍意义。
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