Paudel Keshav Raj, Lee Ung-Won, Kim Dong-Wook
Department of Oriental Medicine Resources, Mokpo National University, Muan-gun, Jeonnam 534-729, South Korea.
Department of Physics, Mokpo National University, Muan-gun, Jeonnam 534-729, South Korea.
J Integr Med. 2016 Mar;14(2):134-42. doi: 10.1016/S2095-4964(16)60249-2.
Hypercholesterolemia is one of the well-established risk factors for cardiovascular mortality and morbidity in coronary heart disease. The aim of this study was to investigate the anti-atherogenic effect of Chungtaejeon (CTJ, a Korean fermented tea) aqueous extract on proliferation and migration of human aortic smooth muscle cells (HASMCs) in vivo and in vitro.
The authors used high-fat atherogenic diet (HFAD) to induce hyperlipidemia in Wistar rats in in vivo animal experiments and used HASMCs for in vitro cell experiments. For the in vitro cell experiment, the proliferation of HASMCs was evaluated using the MTT assay. Similarly, the expression of matrix metalloproteinases (MMPs) in HASMCs was measured using gelatin zymography. Antimigratory activity of CTJ was revealed using the wound-healing model and Boyden 's chamber assay. In the in vivo experiment, CTJ was administered in three different doses for 20 d from the initiation of the HFAD. After 20 d, the serum lipid profile and total lipid contents in liver were measured.
Treatment with CTJ for 24 h dose-dependently inhibited the proliferation and migration of HASMCs and expression of MMP-2 in HASMCs. The oral administration of CTJ at concentrations of 200 and 400 mg/kg decreased the levels of low-density lipoprotein cholesterol, total serum cholesterol and hepatic cholesterol of HFAD-fed rats.
CTJ possessed strong antiproliferative, antimigratory, as well as lipid-lowering activities. Thus, CTJ can be considered as a therapeutic option in the treatment of high-fat diet-induced atherosclerosis.
高胆固醇血症是冠心病中心血管疾病死亡率和发病率已明确的危险因素之一。本研究旨在探讨朝鲜发酵茶（CTJ）水提取物在体内和体外对人主动脉平滑肌细胞（HASMCs）增殖和迁移的抗动脉粥样硬化作用。
在体内动物实验中，作者使用高脂致动脉粥样硬化饮食（HFAD）诱导Wistar大鼠高脂血症，并在体外细胞实验中使用HASMCs。对于体外细胞实验，使用MTT法评估HASMCs的增殖。同样，使用明胶酶谱法测量HASMCs中基质金属蛋白酶（MMPs）的表达。使用伤口愈合模型和Boyden小室试验揭示CTJ的抗迁移活性。在体内实验中，从开始给予HFAD起，以三种不同剂量给予CTJ，持续20天。20天后，测量血清脂质谱和肝脏中的总脂质含量。
用CTJ处理24小时可剂量依赖性地抑制HASMCs的增殖和迁移以及HASMCs中MMP-2的表达。以200和400mg/kg的浓度口服CTJ可降低高脂饮食喂养大鼠的低密度脂蛋白胆固醇、总血清胆固醇和肝胆固醇水平。
CTJ具有很强的抗增殖、抗迁移以及降血脂活性。因此，CTJ可被视为治疗高脂饮食诱导的动脉粥样硬化的一种治疗选择。
