Department of Oriental Medicine Resources, Mokpo National University, Mokpo, South Korea.
Plant Foods Hum Nutr. 2011 Mar;66(1):27-33. doi: 10.1007/s11130-011-0211-y.
Oxidation susceptibility of serum lipid and the proliferation and migration of vascular smooth muscle cells (VSMC) from tunica media to the sub endothelial region are the key steps in the progression of atherosclerosis. The objective of this study was to determine the effects of Chungtaejeon (CTJ) on oxidation and cytokine induced proliferation and migration of human aortic smooth muscle cells (HASMC). The antioxidative effects of CTJ were evaluated by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay, nitric oxide (NO) scavenging assay and thiobarbituric acid reactive substance (TBARS) assay. Similarly, the proliferation, migration and expression of matrix metalloproteinases (MMPs) in HASMC were assessed by MTT assay, transwell Boyden chamber assay and gelatin zymography, respectively. Western blotting was done to determine the protein expression of MMP-9, phospho extracellular regulated kinase (pERK1/2) and phospho c-Jun N-terminal kinase (pJNK). In results, the IC(50) values for DPPH and NO scavenging activities were 8.91 μg/ml and 14.32 μg/ml, respectively. Furthermore, CTJ inhibited TBARS formation dose dependently. The pretreatment of CTJ dose dependently inhibited the tumor necrosis factor-α (TNF-α) induced proliferation and MMP-9 expression and platelet derived growth factor (PDGF) induced migration of HASMC. Thus, CTJ can be suggested to have beneficial effect in the prevention of atherosclerosis.
血清脂质的氧化易感性以及血管平滑肌细胞（VSMC）从中膜向亚内皮区域的增殖和迁移是动脉粥样硬化进展的关键步骤。本研究的目的是确定 Chungtaejeon（CTJ）对人主动脉平滑肌细胞（HASMC）氧化和细胞因子诱导的增殖和迁移的影响。通过 1,1-二苯基-2-苦基肼（DPPH）清除测定法、一氧化氮（NO）清除测定法和硫代巴比妥酸反应物质（TBARS）测定法评估 CTJ 的抗氧化作用。同样，通过 MTT 测定法、Transwell Boyden 室测定法和明胶酶谱法分别评估 HASMC 的增殖、迁移和基质金属蛋白酶（MMPs）的表达。通过 Western blot 测定法确定 MMP-9、磷酸细胞外调节激酶（pERK1/2）和磷酸 c-Jun N-末端激酶（pJNK）的蛋白表达。结果，DPPH 和 NO 清除活性的 IC（50）值分别为 8.91μg/ml 和 14.32μg/ml。此外，CTJ 可剂量依赖性抑制 TBARS 形成。CTJ 的预处理可剂量依赖性抑制肿瘤坏死因子-α（TNF-α）诱导的 HASMC 增殖和 MMP-9 表达以及血小板衍生生长因子（PDGF）诱导的迁移。因此，CTJ 可用于预防动脉粥样硬化。
