Yakubov Gleb E, Bonilla Mauricio R, Chen Huaying, Doblin Monika S, Bacic Antony, Gidley Michael J, Stokes Jason R
Australian Research Council Centre of Excellence in Plant Cell Walls School of Chemical Engineering, The University of Queensland, Queensland, Australia
Australian Research Council Centre of Excellence in Plant Cell Walls School of Chemical Engineering, The University of Queensland, Queensland, Australia.
J Exp Bot. 2016 Apr;67(9):2799-816. doi: 10.1093/jxb/erw117. Epub 2016 Mar 17.
Nanoindentation experiments are performed using an atomic force microscope (AFM) to quantify the spatial distribution of mechanical properties of plant cell walls at nanometre length scales. At any specific location on the cell wall, a complex (non-linear) force-indentation response occurs that can be deconvoluted using a unique multiregime analysis (MRA). This allows an unambiguous evaluation of the local transverse elastic modulus of the wall. Nanomechanical measurements on suspension-cultured cells (SCCs), derived from Italian ryegrass (Lolium multiflorum) starchy endosperm, show three characteristic modes of deformation and a spatial distribution of elastic moduli across the surface. 'Soft' and 'hard' domains are found across length scales between 0.1 µm and 3 µm, which is well above a typical pore size of the polysaccharide mesh. The generality and wider applicability of this mechanical heterogeneity is verified through in planta characterization on leaf epidermal cells of Arabidopsis thaliana and L. multiflorum The outcomes of this research provide a basis for uncovering and quantifying the relationships between local wall composition, architecture, cell growth, and/or morphogenesis.
使用原子力显微镜（AFM）进行纳米压痕实验，以量化植物细胞壁在纳米长度尺度上力学性能的空间分布。在细胞壁的任何特定位置，都会出现复杂的（非线性）力-压痕响应，可使用独特的多区域分析（MRA）进行反卷积。这使得能够明确评估细胞壁的局部横向弹性模量。对源自意大利黑麦草（多花黑麦草）淀粉胚乳的悬浮培养细胞（SCC）进行的纳米力学测量显示出三种特征性变形模式以及整个表面弹性模量的空间分布。在0.1 µm至3 µm的长度尺度上发现了“软”域和“硬”域，这远高于多糖网络的典型孔径。通过对拟南芥和多花黑麦草叶片表皮细胞的植物体内表征，验证了这种机械异质性的普遍性和更广泛的适用性。本研究结果为揭示和量化局部细胞壁组成、结构、细胞生长和/或形态发生之间的关系提供了基础。
