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2015年在加拿大不列颠哥伦比亚省分离出的一种新型重配高致病性H5N1病毒的病理生物学特征

Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.

作者信息

Berhane Yohannes, Kobasa Darwyn, Embury-Hyatt Carissa, Pickering Brad, Babiuk Shawn, Joseph Tomy, Bowes Victoria, Suderman Mathew, Leung Anders, Cottam-Birt Colleen, Hisanaga Tamiko, Pasick John

机构信息

Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.

Department of Animal Science, University of Manitoba, Winnipeg, Canada.

出版信息

Sci Rep. 2016 Mar 18;6:23380. doi: 10.1038/srep23380.

Abstract

In the current study, we describe the pathobiologic characteristics of a novel reassortant virus - A/chicken/BC/FAV-002/2015 (H5N1) belonging to clade 2.3.4.4 that was isolated from backyard chickens in British Columbia, Canada. Sequence analyses demonstrate PB1, PA, NA and NS gene segments were of North American lineage while PB2, HA, NP and M were derived from a Eurasian lineage H5N8 virus. This novel virus had a 19 amino acid deletion in the neuraminidase stalk. We evaluated the pathogenic potential of this isolate in various animal models. The virus was highly pathogenic to mice with a LD50 of 10 plaque forming units (PFU), but had limited tissue tropism. It caused only subclinical infection in pigs which did result in seroconversion. This virus was highly pathogenic to chickens, turkeys, juvenile Muscovy ducks (Cairnia moschata foma domestica) and adult Chinese geese (Anser cynoides domesticus) causing a systemic infection in all species. The virus was also efficiently transmitted and resulted in mortality in naïve contact ducks, geese and chickens. Our findings indicate that this novel H5N1 virus has a wide host range and enhanced surveillance of migratory waterfowl may be necessary in order to determine its potential to establish itself in the wild bird reservoir.

摘要

在本研究中,我们描述了一种新型重配病毒——A/鸡/BC/FAV-002/2015(H5N1)的病理生物学特征,该病毒属于2.3.4.4分支,是从加拿大不列颠哥伦比亚省的后院鸡中分离出来的。序列分析表明,PB1、PA、NA和NS基因片段属于北美谱系,而PB2、HA、NP和M则源自欧亚谱系的H5N8病毒。这种新型病毒的神经氨酸酶柄部有19个氨基酸缺失。我们在各种动物模型中评估了该分离株的致病潜力。该病毒对小鼠具有高致病性,半数致死剂量(LD50)为10个蚀斑形成单位(PFU),但组织嗜性有限。它在猪中仅引起亚临床感染,但确实导致了血清转化。这种病毒对鸡、火鸡、幼年番鸭(Cairnia moschata foma domestica)和成年中国鹅(Anser cynoides domesticus)具有高致病性,在所有物种中均引起全身感染。该病毒还能有效传播,并导致未接触过该病毒的接触鸭、鹅和鸡死亡。我们的研究结果表明,这种新型H5N1病毒具有广泛的宿主范围,可能有必要加强对候鸟的监测,以确定其在野生鸟类宿主中定殖的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff47/4796896/16978993fe23/srep23380-f1.jpg

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