在尼泊尔检测ST772型杀白细胞素阳性的耐甲氧西林金黄色葡萄球菌（孟加拉湾克隆株）以及具有弹性蛋白结合蛋白基因变异体的ST22型金黄色葡萄球菌分离株。
Detection of ST772 Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus (Bengal Bay clone) and ST22 S. aureus isolates with a genetic variant of elastin binding protein in Nepal.
作者信息
Pokhrel R H, Aung M S, Thapa B, Chaudhary R, Mishra S K, Kawaguchiya M, Urushibara N, Kobayashi N
机构信息
Genesis Laboratory and Research, Tribhuvan University, Kathmandu, Nepal.
Sapporo Medical University, Sapporo, Japan.
出版信息
New Microbes New Infect. 2016 Feb 18;11:20-7. doi: 10.1016/j.nmni.2016.02.001. eCollection 2016 May.
Genetic characteristics were analysed for recent clinical isolates of methicillin-resistant and -susceptible Staphylococcus aureus (MRSA and MSSA respectively) in Kathmandu, Nepal. MRSA isolates harbouring Panton-Valentine leukocidin (PVL) genes were classified into ST1, ST22 and ST88 with SCCmec-IV and ST772 with SCCmec-V (Bengal Bay clone), while PVL-positive MSSA into ST22, ST30 and ST772. ST22 isolates (PVL-positive MRSA and MSSA, PVL-negative MRSA) possessed a variant of elastin binding protein gene (ebpS) with an internal deletion of 180 bp, which was similar to that reported for ST121 S. aureus previously outside Nepal. Phylogenetic analysis indicated that the ebpS variant in ST22 might have occurred independently of ST121 strains. This is the first report of ST772 PVL-positive MRSA in Nepal and detection of the deletion variant of ebpS in ST22 S. aureus.
对尼泊尔加德满都近期耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）的临床分离株进行了遗传特征分析。携带杀白细胞素（PVL）基因的MRSA分离株，携带SCCmec-IV的被分类为ST1、ST22和ST88，携带SCCmec-V的（孟加拉湾克隆）被分类为ST772，而PVL阳性的MSSA被分类为ST22、ST30和ST772。ST22分离株（PVL阳性MRSA和MSSA、PVL阴性MRSA）具有弹性蛋白结合蛋白基因（ebpS）的一个变体，其内部缺失180bp，这与之前在尼泊尔境外报道的ST121金黄色葡萄球菌的情况相似。系统发育分析表明，ST22中的ebpS变体可能独立于ST121菌株出现。这是尼泊尔关于ST772 PVL阳性MRSA的首次报告，也是在ST22金黄色葡萄球菌中检测到ebpS缺失变体的报告。
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