用于耐万古霉素肠球菌监测分离株的简单可靠的多重聚合酶链反应检测法。
Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci.
作者信息
Kariyama R, Mitsuhata R, Chow J W, Clewell D B, Kumon H
机构信息
Section of Infection Control, Okayama University Hospital, Okayama 700-8558, Japan.
出版信息
J Clin Microbiol. 2000 Aug;38(8):3092-5. doi: 10.1128/JCM.38.8.3092-3095.2000.
Abstract
Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.
摘要
已优化条件，以便在医院监测样本中使用多重聚合酶链反应（PCR）检测耐万古霉素肠球菌。在一个反应管中使用了七组引物，分别靶向vanA、vanB、vanC1、vanC2/C3基因、粪肠球菌特异性基因、屎肠球菌特异性基因和rrs（16S核糖体RNA）基因。本研究开发的PCR方法简单可靠，可快速鉴定耐万古霉素肠球菌。
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