Ghalavand Zohreh, Alebouyeh Masoud, Ghanati Kiandokht, Azimi Leila, Rashidan Marjan
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pediatric Infections Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gut Pathog. 2020 Sep 9;12:42. doi: 10.1186/s13099-020-00380-7. eCollection 2020.
Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired isolated from the urine of symptomatic patients and their fecal samples.
Out of the studied patients, 63 pairs of isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤ 4 µg/ml), while resistance to tetracycline (urine: 88.9%; stool: 76.2%) and minocycline (urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included , , and . RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively.
Similarity of strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenic strains.
社区获得性尿路感染（CA-UTI）可能由内源性或外源性途径引起。为了揭示这种关系，我们调查了从有症状患者尿液及其粪便样本中分离出的配对菌株的分子指纹和基因型。
在所研究的患者中，从其尿液和粪便样本中同时获得了63对菌株分离物。所有菌株对万古霉素、利奈唑胺、呋喃妥因和达托霉素敏感（MIC值：≤4μg/ml），而大多数菌株对四环素（尿液：88.9%；粪便：76.2%）和米诺环素（尿液：87.3%，粪便：71.4%）耐药。检测到的最常见毒力基因包括、和。RAPD-PCR和PFGE分析分别显示，在26.9%和15.8%的患者中，配对菌株的分子指纹模式相同。
尿液和粪便样本中菌株的相似性证实了通过肠道定植菌污染发生内源性感染。在致病菌株中携带完整的毒力基因型与内源性UTI在统计学上相关，这表明它们可能参与了尿路致病性菌株的致病性。
