Carbone Marilena, Sabbatella Gianfranco, Antonaroli Simonetta, Orlando Viviana, Biagioni Stefano, Nucara Alessandro
Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy.
Consorzio Interuniversitario Biostrutture e Biosistemi, Viale Medaglie d'Oro 305, 00136, Rome, Italy.
Eur Biophys J. 2016 Sep;45(6):565-71. doi: 10.1007/s00249-016-1122-5. Epub 2016 Mar 26.
A proton caged compound, the 1-(2-nitrophenyl)- ethylhexadecyl sulfonate (HDNS), was dosed into HEK-293 at different incubation times. Samples were irradiated with filtered UV light for inducing photolysis of the HDNS and then probed by infrared spectroscopy. The intracellular acidification reaction can be followed by monitoring the consequent CO2 peak intensity variation. The total CO2 produced is similar for all the samples, hence it is only a function of the initial HDNS concentration. The way it is achieved, though, is different for the different incubation times and follows kinetics, which results in a combination of a linear CO2 increase and a steep CO2 increase followed by a decay. This is interpreted in terms of confinement of the HDNS into intracellular vesicles of variable average size and sensitive to UV light when they reach critical dimensions.
一种质子笼化合物，1-(2-硝基苯基)-十六烷基磺酸乙酯(HDNS)，在不同的孵育时间加入到HEK-293细胞中。用过滤后的紫外光照射样品以诱导HDNS的光解，然后用红外光谱进行探测。通过监测随之而来的二氧化碳峰强度变化可以追踪细胞内酸化反应。所有样品产生的总二氧化碳量相似，因此它只是初始HDNS浓度的函数。然而，实现这一过程的方式因孵育时间不同而不同，并且遵循动力学，这导致二氧化碳呈线性增加和急剧增加，随后衰减。这可以解释为HDNS被限制在平均大小可变的细胞内囊泡中，当它们达到临界大小时对紫外光敏感。
