Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, People's Republic of China.
Biofunctional Molecule Exploratory (BMEX) Research Group, School of Pharmacy, Monash University Malaysia, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
BMC Microbiol. 2019 Feb 13;19(1):38. doi: 10.1186/s12866-019-1409-7.
Colon cancer is the third most commonly diagnosed cancer worldwide, with a commensurately high mortality rate. The search for novel antioxidants and specific anticancer agents which may inhibit, delay or reverse the development of colon cancer is thus an area of great interest; Streptomyces bacteria have been demonstrated to be a source of such agents.
The extract from Streptomyces sp. MUM265- a strain which was isolated and identified from Kuala Selangor mangrove forest, Selangor, Malaysia- was analyzed and found to exhibit antioxidant properties as demonstrated via metal-chelating ability as well as superoxide anion, DPPH and ABTS radical scavenging activities. This study also showed that MUM265 extract demonstrated cytotoxicity against colon cancer cells as evidenced by the reduced cell viability of Caco-2 cell line. Treatment with MUM265 extract induced depolarization of mitochondrial membrane potential and accumulation of subG cells in cell cycle analysis, suggesting that MUM265 exerted apoptosis-inducing effects on Caco-2 cells.
These findings indicate that mangrove derived Streptomyces sp. MUM265 represents a valuable bioresource of bioactive compounds for the future development of chemopreventive agents, with particular promise suggested for treatment of colon cancer.
结肠癌是全球第三大常见癌症，死亡率也相应较高。因此，寻找新型抗氧化剂和特定的抗癌药物来抑制、延缓或逆转结肠癌的发展是一个非常有意义的领域；链霉菌已被证明是此类药物的来源。
从马来西亚雪兰莪州瓜拉雪兰莪红树林中分离和鉴定的链霉菌菌株 MUM265 的提取物表现出抗氧化特性，如金属螯合能力以及超氧阴离子、DPPH 和 ABTS 自由基清除活性所证明的那样。这项研究还表明，MUM265 提取物对结肠癌细胞具有细胞毒性作用，这可从 Caco-2 细胞系的细胞活力降低得到证明。用 MUM265 提取物处理导致线粒体膜电位去极化和细胞周期分析中亚 G 期细胞的积累，表明 MUM265 对 Caco-2 细胞具有诱导凋亡的作用。
这些发现表明，来源于红树林的链霉菌菌株 MUM265 代表了具有生物活性化合物的有价值的生物资源，可用于未来开发化学预防剂，特别是在结肠癌的治疗方面具有很大的潜力。
