Bansho Yohsuke, Furubayashi Taro, Ichihashi Norikazu, Yomo Tetsuya
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan; Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-0083, Japan;
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan;
Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):4045-50. doi: 10.1073/pnas.1524404113. Epub 2016 Mar 28.
To date, various cellular functions have been reconstituted in vitro such as self-replication systems using DNA, RNA, and proteins. The next important challenges include the reconstitution of the interactive networks of self-replicating species and investigating how such interactions generate complex ecological behaviors observed in nature. Here, we synthesized a simple replication system composed of two self-replicating host and parasitic RNA species. We found that the parasitic RNA eradicates the host RNA under bulk conditions; however, when the system is compartmentalized, a continuous oscillation pattern in the population dynamics of the two RNAs emerges. The oscillation pattern changed as replication proceeded mainly owing to the evolution of the host RNA. These results demonstrate that a cell-like compartment plays an important role in host-parasite ecological dynamics and suggest that the origin of the host-parasite coevolution might date back to the very early stages of the evolution of life.
迄今为止，各种细胞功能已在体外重建，例如使用DNA、RNA和蛋白质的自我复制系统。接下来的重要挑战包括重建自我复制物种的交互网络，并研究此类相互作用如何产生自然界中观察到的复杂生态行为。在此，我们合成了一个由两种自我复制的宿主RNA和寄生RNA物种组成的简单复制系统。我们发现，在大量条件下寄生RNA会根除宿主RNA；然而，当系统被区室化时，两种RNA种群动态中会出现连续振荡模式。随着复制的进行，振荡模式发生了变化，这主要是由于宿主RNA的进化。这些结果表明，类似细胞的区室在宿主-寄生虫生态动态中起着重要作用，并表明宿主-寄生虫共同进化的起源可能可追溯到生命进化的非常早期阶段。
