静脉注射后大鼠体内二氧化钛纳米颗粒的生物分布与清除
Biodistribution and Clearance of TiO2 Nanoparticles in Rats after Intravenous Injection.
作者信息
Elgrabli Dan, Beaudouin Remy, Jbilou Nawel, Floriani Magali, Pery Alexandre, Rogerieux Françoise, Lacroix Ghislaine
机构信息
Institut National de l'Environnement Industriel et des Risques (INERIS), Parc technologique Alata, Verneuil en Halatte, France; Laboratoire Matières et systèmes complexes, UMR7057 CNRS/Université paris Diderot, Paris, France.
Institut National de l'Environnement Industriel et des Risques (INERIS), Parc technologique Alata, Verneuil en Halatte, France.
出版信息
PLoS One. 2015 Apr 24;10(4):e0124490. doi: 10.1371/journal.pone.0124490. eCollection 2015.
Titanium dioxide (TiO2) nanoparticles are used in many applications. Due to their small size, easy body penetration and toxicological adverse effects have been suspected. Numerous studies have tried to characterize TiO2 translocation after oral, dermal or respiratory exposure. In this study, we focused on TiO2 nanoparticle biodistribution, clearance and toxicological effects after intravenous injection, considering TiO2 translocation in the blood occurs. Using ICP-OES, transmission electron microscopy, and histological methods, we found TiO2 accumulation in liver, lungs and spleen. We estimated TiO2 nanoparticles' half life in the body to about 10 days. Clinical biomarkers were also quantified for 56 days to identify potential toxicological impact on lungs, blood, liver, spleen and kidneys. Results showed absence of toxicological effects after TiO2 intravenous injection at concentrations of 7.7 to 9.4 mg/kg.
二氧化钛（TiO₂）纳米颗粒有许多应用。因其尺寸小，人们怀疑它容易穿透人体并产生毒理学不良反应。众多研究试图描述口服、经皮或呼吸道接触二氧化钛后的转运情况。在本研究中，鉴于二氧化钛在血液中会发生转运，我们重点研究了静脉注射后二氧化钛纳米颗粒的生物分布、清除及毒理学效应。通过电感耦合等离子体发射光谱法（ICP - OES）、透射电子显微镜和组织学方法，我们发现二氧化钛在肝脏、肺和脾脏中蓄积。我们估计二氧化钛纳米颗粒在体内的半衰期约为10天。我们还对临床生物标志物进行了56天的定量分析，以确定对肺、血液、肝脏、脾脏和肾脏的潜在毒理学影响。结果显示，静脉注射浓度为7.7至9.4 mg/kg的二氧化钛后未出现毒理学效应。
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