Takebayashi M
First Department of Surgery, Tottori University School of Medicine, Yonago, Japan.
Nihon Geka Gakkai Zasshi. 1989 Jan;90(1):49-58.
The promoting effect of bile acids on the development of gastric carcinoma was examined in rats treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). At the first experiment, two hundred and fifteen male Wistar rats were divided into 5 groups; after oral administration MNNG (120 micrograms/ml) for 24 weeks, group 1 received tap water, group 2 administered of chenodeoxycholic acid (CDCA) solution, group 3 had deoxycholic acid (DCA) solution for the next 12 weeks. Group 4 received CDCA solution and group 5 received DCA solution for 36 weeks without MNNG. At the second experiment, fifty one rats were divided into 3 groups; for the first 12 weeks, group 1 received tap water, group 2 CDCA and group 3 DCA. These 3 groups received MNNG for the next 24 weeks followed by tap water for 12 weeks. The incidence of gastric adenocarcinoma in MNNG-treated rats was significantly higher in group 3 (63.6%) as compared with that in group 1 (36.7%) in the first experiment. No carcinoma lesions was found in groups 4 and 5. In the second experiment, no significant changes was observed among 3 groups. Undifferentiated adenocarcinomas were identified in groups 2 and 3, especially treated with MNNG plus bile acids. The result suggested a promoting effect of bile acids, especially DCA, in stomach carcinogenesis.
在经N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理的大鼠中，研究了胆汁酸对胃癌发生发展的促进作用。在第一个实验中，将215只雄性Wistar大鼠分为5组；在口服MNNG（120微克/毫升）24周后，第1组给予自来水，第2组给予鹅去氧胆酸（CDCA）溶液，第3组在接下来的12周给予脱氧胆酸（DCA）溶液。第4组给予CDCA溶液，第5组在无MNNG的情况下给予DCA溶液36周。在第二个实验中，将51只大鼠分为3组；在最初的12周，第1组给予自来水，第2组给予CDCA，第3组给予DCA。这3组在接下来的24周接受MNNG，随后12周给予自来水。在第一个实验中，MNNG处理的大鼠中，第3组胃腺癌的发生率（63.6%）显著高于第1组（36.7%）。第4组和第5组未发现癌性病变。在第二个实验中，3组之间未观察到显著变化。在第2组和第3组中发现了未分化腺癌，尤其是在MNNG加胆汁酸处理的情况下。结果表明胆汁酸，尤其是DCA，在胃癌发生过程中具有促进作用。
