Nair Amruta, Balasaravanan T, Malik S V S, Mohan Vysakh, Kumar Manesh, Vergis Jess, Rawool Deepak B
Division of Veterinary Public Health, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
Department of Biotechnology, Nehru Arts & Science College, Bharathiar University, Coimbatore, Tamil Nadu, India.
Vet World. 2015 May;8(5):669-73. doi: 10.14202/vetworld.2015.669-673. Epub 2015 May 27.
This study was carried out to determine the prevalence, distribution, and identification of Salmonella serotypes in diarrheagenic infants and young animals, including sewage waste and fresh vegetables.
A total of 550 samples were processed for the isolation of Salmonella spp., using standard microbiological and biochemical tests. Further polymerase chain reaction (PCR) detection of Salmonella genus was carried out using self-designed primers targeting invA gene and thereafter identification of important serotypes namely Salmonella Enterica serovar Typhimurium, Salmonella Enterica serovar Enteritidis, Salmonella Enterica serovar Typhi was performed using published standardized multiplex PCR.
An overall low prevalence of 2.5% (14/550) was observed. The observed prevalence of Salmonella spp. in diarrheagenic infants was 1.2% (05/400), diarrheagenic young animals 4% (02/50), sewage waste 10% (05/50), and fresh vegetables 4% (02/50), respectively. In diarrheagenic infants, of the five Salmonella isolates identified, two were Salmonella Typhimurium, two Salmonella Enteritidis, and one was unidentified and hence designated as other Salmonella serovar. All the Salmonella isolates identified from diarrheagenic young animals and sewage waste belonged to other Salmonella serovar, whereas, of the two isolates recovered from fresh vegetables, one was identified as other Salmonella serovar, and one as Salmonella Typhimurium, respectively.
Isolation of Salmonella spp. especially from sewage waste and fresh vegetable is a matter of great concern from public health point of view because these sources can accidentally serve as a potential vehicle for transmission of Salmonella spp. to animals and human beings.
开展本研究以确定腹泻性婴幼儿及幼龄动物，包括污水废物和新鲜蔬菜中沙门氏菌血清型的流行情况、分布及鉴定。
共处理550份样本以分离沙门氏菌属，采用标准微生物学和生化试验。使用针对invA基因的自行设计引物对沙门氏菌属进行进一步的聚合酶链反应（PCR）检测，之后使用已发表的标准化多重PCR对重要血清型即肠炎沙门氏菌鼠伤寒血清型、肠炎沙门氏菌肠炎血清型、伤寒沙门氏菌进行鉴定。
总体流行率较低，为2.5%（14/550）。腹泻性婴幼儿中沙门氏菌属的流行率为1.2%（05/400），腹泻性幼龄动物为4%（02/50），污水废物为10%（05/50），新鲜蔬菜为4%（02/50）。在腹泻性婴幼儿中，鉴定出的5株沙门氏菌分离株中，2株为鼠伤寒沙门氏菌，2株为肠炎沙门氏菌，1株未鉴定出来，因此指定为其他沙门氏菌血清型。从腹泻性幼龄动物和污水废物中鉴定出的所有沙门氏菌分离株均属于其他沙门氏菌血清型，而从新鲜蔬菜中回收的2株分离株中，1株鉴定为其他沙门氏菌血清型，1株为鼠伤寒沙门氏菌。
从公共卫生角度来看，分离出沙门氏菌属，尤其是从污水废物和新鲜蔬菜中分离出，是一个非常令人担忧的问题，因为这些来源可能意外地成为沙门氏菌属向动物和人类传播的潜在载体。
